Evaluation and Correlation of 17β-Estradiol in Blood and Estrogen Receptor α (ERα) and Estrogen Receptor β(ER β) in Tissue of Oral Squamous Cell Carcinoma.

Q2 Medicine

Asian Pacific Journal of Cancer Prevention Pub Date : 2025-05-01 DOI:10.31557/APJCP.2025.26.5.1735

Eesha Thakare, Minal Chaudhary, Madhuri Gawande, Amol Ramchandra Gadbail, Prajakta Zade

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Abstract

Objective: Evaluation and correlation of 17β-Estradiol(E2) in blood and Estrogen Receptor α (ERα) and Estrogen Receptor β(ER β) in tissue of four groups divided as normal control subjects (group I), active control subjects (group II), premalignant(leukoplakia) (group III) and OSCC patients (Group IV).

Methods: Subjects were divided in four groups mentioned previously and evaluated individually for 17β-estradiol(E2) in blood and ERα & ER β in tissue. The blood samples were evaluated by chemiluminescence assay and tissues samples were evaluated by Real-Time PCR for tissue analysis. Analysis of variance (ANOVA) was applied for quantitative evaluation of variables in each group followed by TukeyB test. Pearson Correlation Coefficient was used to study the correlation between the variables. Survival analysis was calculated by Kaplan-Meier Survival Analysis.

Results: The results are indicative of statistically significant increased levels of E2 in the 4 groups by analysis of variance (p value-0.0327) followed by TukeyB test with statistically significant difference between Group VI and I (sig value-0.008) and Group VI and II (sig value-0.029). The Pearson correlation coefficient demonstrated that the mean levels of Estradiol are positively correlated with mean levels of ER α (p value- 0.030). Results did not indicate an association between survival and expression of E2, ER α and ER β.

Conclusion: The levels of E2 can be used as a marker in predicting the progression of disease from normal tissue not exposed to tobacco to normal tissue exposed to tobacco to premalignant to OSCC. The ERα in tissue is positively correlated with the increased levels of E2 in serum, so ERα expression in tissue along with E2 in serum could be used to identify the subsets of patients with higher risk of developing OSCC, especially those subjects with established tobacco habit but no appreciable change in oral mucosa.

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口腔鳞状细胞癌血液中17β-雌二醇与组织中雌激素受体α (ERα)和雌激素受体β(ER β)的相关性研究

目的：评价正常对照组（ⅰ组）、活性对照组（ⅱ组）、癌前病变（白斑）组（ⅲ组）和OSCC患者（ⅳ组）血液中17β-雌二醇（E2）与组织中雌激素受体α (ERα)和雌激素受体β(ER β)水平的相关性。方法：将受试者分为上述四组，分别测定血液中17β-雌二醇（E2）水平和组织中ERα、ER β水平。采用化学发光法评价血液样本，Real-Time PCR评价组织样本进行组织分析。采用方差分析（ANOVA）对各组变量进行定量评价，并进行TukeyB检验。Pearson相关系数用于研究变量之间的相关性。生存分析采用Kaplan-Meier生存分析。结果：经方差分析（p值0.0327）和TukeyB检验，结果显示4组患者E2水平升高有统计学意义，其中组VI与组I （sig值0.008）、组VI与组II （sig值0.029）差异有统计学意义。Pearson相关系数显示雌二醇平均水平与ER α平均水平呈正相关（p值- 0.030）。结果未显示生存与E2、ER α和ER β的表达相关。结论：E2水平可作为预测未接触烟草的正常组织到接触烟草的正常组织、癌前病变到OSCC进展的标志。组织中ERα与血清中E2水平升高呈正相关，因此组织中ERα与血清中E2的表达可用于识别OSCC高危患者亚群，特别是那些有吸烟习惯但口腔黏膜无明显变化的患者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Pacific Journal of Cancer Prevention 医学-肿瘤学

CiteScore

2.80

自引率

0.00%

发文量

779

审稿时长

3 months

期刊介绍： Cancer is a very complex disease. While many aspects of carcinoge-nesis and oncogenesis are known, cancer control and prevention at the community level is however still in its infancy. Much more work needs to be done and many more steps need to be taken before effective strategies are developed. The multidisciplinary approaches and efforts to understand and control cancer in an effective and efficient manner, require highly trained scientists in all branches of the cancer sciences, from cellular and molecular aspects to patient care and palliation. The Asia Pacific Organization for Cancer Prevention (APOCP) and its official publication, the Asia Pacific Journal of Cancer Prevention (APJCP), have served the community of cancer scientists very well and intends to continue to serve in this capacity to the best of its abilities. One of the objectives of the APOCP is to provide all relevant and current scientific information on the whole spectrum of cancer sciences. They aim to do this by providing a forum for communication and propagation of original and innovative research findings that have relevance to understanding the etiology, progression, treatment, and survival of patients, through their journal. The APJCP with its distinguished, diverse, and Asia-wide team of editors, reviewers, and readers, ensure the highest standards of research communication within the cancer sciences community across Asia as well as globally. The APJCP publishes original research results under the following categories: -Epidemiology, detection and screening. -Cellular research and bio-markers. -Identification of bio-targets and agents with novel mechanisms of action. -Optimal clinical use of existing anti-cancer agents, including combination therapies. -Radiation and surgery. -Palliative care. -Patient adherence, quality of life, satisfaction. -Health economic evaluations.