KS-NailMel-1: a novel cell line of nail apparatus melanoma.

IF 3.4 3区生物学 Q3 CELL BIOLOGY

Human Cell Pub Date : 2025-05-28 DOI:10.1007/s13577-025-01242-7

Takamichi Ito, Yuka Tanaka, Keiko Tanegashima, Kiichiro Nishio, Hiroki Hashimoto, Toshio Ichiki, Fumitaka Ohno, Yumiko Kaku-Ito, Takeshi Nakahara

{"title":"KS-NailMel-1: a novel cell line of nail apparatus melanoma.","authors":"Takamichi Ito, Yuka Tanaka, Keiko Tanegashima, Kiichiro Nishio, Hiroki Hashimoto, Toshio Ichiki, Fumitaka Ohno, Yumiko Kaku-Ito, Takeshi Nakahara","doi":"10.1007/s13577-025-01242-7","DOIUrl":null,"url":null,"abstract":"<p><p>Nail apparatus melanoma (NAM) is a specific type of cutaneous melanoma that develops in the nail apparatus of the hands and feet. The prognosis for metastatic NAM is poor due to a lack of fully effective systemic therapies. However, the difficulty in obtaining a NAM model has hindered basic research aimed at discovering effective treatment strategies. In this study, we established a NAM cell line, named KS-NailMel-1, from a primary tumor located on the nail apparatus of the left ring finger of a 68-year-old Japanese female. The cells were successfully maintained for over 9 months, exhibiting a doubling time of 38.6 ± 1.94 h. KS-NailMel-1 displayed consistent growth, spheroid formation, and invasiveness, and was confirmed to be identical to the original tumor through short tandem repeat analyses, whole-exome sequencing, and immunohistochemistry. Western blotting of the cells demonstrated the protein expression of NECTIN4, which has recently attracted attention as a potential therapeutic target for melanoma. The KS-NailMel-1 cell line represents a valuable resource for basic and preclinical research on NAM, deepening our understanding of the tumor characteristics and facilitating the development of treatment strategies for this rare form of cancer.</p>","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"112"},"PeriodicalIF":3.4000,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119781/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Human Cell","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s13577-025-01242-7","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Nail apparatus melanoma (NAM) is a specific type of cutaneous melanoma that develops in the nail apparatus of the hands and feet. The prognosis for metastatic NAM is poor due to a lack of fully effective systemic therapies. However, the difficulty in obtaining a NAM model has hindered basic research aimed at discovering effective treatment strategies. In this study, we established a NAM cell line, named KS-NailMel-1, from a primary tumor located on the nail apparatus of the left ring finger of a 68-year-old Japanese female. The cells were successfully maintained for over 9 months, exhibiting a doubling time of 38.6 ± 1.94 h. KS-NailMel-1 displayed consistent growth, spheroid formation, and invasiveness, and was confirmed to be identical to the original tumor through short tandem repeat analyses, whole-exome sequencing, and immunohistochemistry. Western blotting of the cells demonstrated the protein expression of NECTIN4, which has recently attracted attention as a potential therapeutic target for melanoma. The KS-NailMel-1 cell line represents a valuable resource for basic and preclinical research on NAM, deepening our understanding of the tumor characteristics and facilitating the development of treatment strategies for this rare form of cancer.

查看原文本刊更多论文

KS-NailMel-1：甲器黑色素瘤的新细胞系。

指甲器官黑色素瘤（NAM）是一种特殊类型的皮肤黑色素瘤，发生在手和脚的指甲器官。由于缺乏完全有效的全身治疗，转移性不正性的预后很差。然而，获得非典型肺炎模型的困难阻碍了旨在发现有效治疗策略的基础研究。在这项研究中，我们从一名68岁的日本女性左无名指指甲器官的原发肿瘤中建立了一个命名为KS-NailMel-1的NAM细胞系。KS-NailMel-1细胞存活时间为38.6±1.94 h，维持时间超过9个月。通过短串联重复序列分析、全外显子组测序和免疫组化，证实KS-NailMel-1与原始肿瘤相同，具有稳定的生长、球形形成和侵袭性。细胞的Western blotting证实了NECTIN4的蛋白表达，该蛋白最近作为黑色素瘤的潜在治疗靶点引起了人们的关注。KS-NailMel-1细胞系为NAM的基础和临床前研究提供了宝贵的资源，加深了我们对肿瘤特征的理解，促进了这种罕见癌症治疗策略的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Human Cell CELL BIOLOGY-

CiteScore

5.90

自引率

2.30%

发文量

176

审稿时长

4.5 months

期刊介绍： Human Cell is the official English-language journal of the Japan Human Cell Society. The journal serves as a forum for international research on all aspects of the human cell, encompassing not only cell biology but also pathology, cytology, and oncology, including clinical oncology. Embryonic stem cells derived from animals, regenerative medicine using animal cells, and experimental animal models with implications for human diseases are covered as well. Submissions in any of the following categories will be considered: Research Articles, Cell Lines, Rapid Communications, Reviews, and Letters to the Editor. A brief clinical case report focusing on cellular responses to pathological insults in human studies may also be submitted as a Letter to the Editor in a concise and short format. Not only basic scientists but also gynecologists, oncologists, and other clinical scientists are welcome to submit work expressing new ideas or research using human cells.