Clastogenic, aneugenic and cytostatic effects of Smallanthus sonchifolius leaf extract in a human tumoral cell line.

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2025-05-29 DOI:10.1080/14786419.2025.2508830

Rocio Ayelen Moreira Szokalo, Marcela López Nigro, Liliana Muschietti, Marcela González Cid, Marta Ana Carballo

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引用次数: 0

Abstract

Smallanthus sonchifolius leaf extracts show potential health benefits but also exhibit cytotoxicity in vitro. This study evaluated the genotoxic effects of a crude extract rich in sesquiterpene lactones (CLM) in HeLa cells. HPLC/MS-MS analysis identified smallanthin, polymatin B, uvedalin, and enhydrin as major components. MTT assay determined the effective concentration range, and the cell cycle analysis was performed using flow cytometry. Genotoxic mechanisms were examined by chromosome aberration analysis and the cytochalasin B-blocked micronucleus with γ-H2AX detection test. The CLM extract induced cytotoxicity, arrested the cell cycle at the G2/M phases and decreased Mitotic and Nuclear Division Indices, and triggered DNA double-strand breaks in DNA and led to chromosome missegregation. Our findings indicate that CLM exerts clastogenic and aneugenic effects, leading to G2 phase arrest for DNA repair. Due to their genotoxicity, STLs show promise as potential candidates for developing new cancer therapeutics.

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小红花叶提取物对人肿瘤细胞系的致裂、非优生和细胞抑制作用。

小红花叶提取物显示潜在的健康益处，但也表现出体外细胞毒性。本研究评估了富含倍半萜内酯（CLM）的粗提取物对HeLa细胞的遗传毒性作用。HPLC/MS-MS分析鉴定出其主要成分为小草氨酸、多聚马苷B、苦藤苷和乙醇。MTT法确定有效浓度范围，流式细胞术进行细胞周期分析。采用染色体畸变分析和γ-H2AX检测细胞松弛素b阻断微核，探讨其遗传毒性机制。CLM提取物诱导细胞毒性，使细胞周期停留在G2/M期，降低有丝分裂和核分裂指数，引发DNA双链断裂，导致染色体错分离。我们的研究结果表明，CLM具有致裂性和非优生作用，导致DNA修复的G2期阻滞。由于其遗传毒性，stl显示出开发新的癌症治疗方法的潜在候选者的希望。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.