{"title":"Evaluation of <i>TaMFT-3A</i> and <i>TaMKK3-4A</i> alleles on wheat pre-harvest sprouting.","authors":"Bo-Wen Zhang, Bai-Song Yang, Xiao-Neng Wan, Xin Ma, Kai-Di Lyu, Han Wang, Shu-Ying Yang, Hui-Hui Zhang, Shu-Nv Hao, Jian Ma, Guo-Zhong Sun","doi":"10.3389/fpls.2025.1594385","DOIUrl":null,"url":null,"abstract":"<p><p>Pre-harvest sprouting (PHS) is a significant challenge affecting global production of wheat (<i>Triticum aestivum</i> L.). Resistance to PHS is governed by both genetic and environmental factors, making reliable molecular markers essential for enhancing PHS resistance through molecular marker-assisted selection (MAS). Genes <i>TaMFT-3A</i> and <i>TaMKK3-4A</i> have been cloned in wheat and are known to regulate PHS resistance in white-grained varieties. In this study, we assessed the allelic variations in these genes and their combined effects on PHS resistance using two recombinant inbred line (RIL) populations, Wanxianbaimaizi/Zhongyou 9507 (WZ) and Wanxianbaimaizi/Jing 411 (WJ), under two distinct field environmental conditions. PHS resistance was assessed by measuring seed germination in physiologically mature stage, correlation and ANOVA were used to analyze PHS data. The germination percentage (GP) and germination index (GI) were significantly correlated across both RIL populations. Specific allelic variations at positions -222, +646, and +666 in the <i>TaMFT-3A</i> gene strongly correlated with PHS resistance. The CGA haplotype at these loci was linked to the highest resistance, while the TAA haplotype was associated with the lowest resistance levels. Additionally, haplotype variation at the +660 locus of <i>TaMKK3-4A</i> demonstrated a weak but environmentally modulated correlation with PHS resistance. This study provides a theoretical foundation for utilizing <i>TaMFT-3A</i> and <i>TaMKK3-4A</i> in molecular breeding strategies to enhance wheat resilience to PHS.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1594385"},"PeriodicalIF":4.1000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116531/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2025.1594385","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Pre-harvest sprouting (PHS) is a significant challenge affecting global production of wheat (Triticum aestivum L.). Resistance to PHS is governed by both genetic and environmental factors, making reliable molecular markers essential for enhancing PHS resistance through molecular marker-assisted selection (MAS). Genes TaMFT-3A and TaMKK3-4A have been cloned in wheat and are known to regulate PHS resistance in white-grained varieties. In this study, we assessed the allelic variations in these genes and their combined effects on PHS resistance using two recombinant inbred line (RIL) populations, Wanxianbaimaizi/Zhongyou 9507 (WZ) and Wanxianbaimaizi/Jing 411 (WJ), under two distinct field environmental conditions. PHS resistance was assessed by measuring seed germination in physiologically mature stage, correlation and ANOVA were used to analyze PHS data. The germination percentage (GP) and germination index (GI) were significantly correlated across both RIL populations. Specific allelic variations at positions -222, +646, and +666 in the TaMFT-3A gene strongly correlated with PHS resistance. The CGA haplotype at these loci was linked to the highest resistance, while the TAA haplotype was associated with the lowest resistance levels. Additionally, haplotype variation at the +660 locus of TaMKK3-4A demonstrated a weak but environmentally modulated correlation with PHS resistance. This study provides a theoretical foundation for utilizing TaMFT-3A and TaMKK3-4A in molecular breeding strategies to enhance wheat resilience to PHS.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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