Biomarker identification associated with M2 tumor-associated macrophage infiltration in glioblastoma.

IF 2.7 3区医学 Q2 CLINICAL NEUROLOGY

Frontiers in Neurology Pub Date : 2025-05-14 eCollection Date: 2025-01-01 DOI:10.3389/fneur.2025.1545608

Xue-Yuan Li, Zhi-Yun Yu, Hong-Jiang Li, Dong-Ming Yan, Chao Yang, Xian-Zhi Liu

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引用次数: 0

Abstract

Purpose: M2 phenotype tumor-associated macrophages (TAMs) can promote tumor growth, invasion, chemotherapy resistance and so on, leading to malignant progression. The aim of this study was to identify novel prognostic profiles in glioblastoma (GBM) by integrating single-cell RNA sequencing (scRNA-seq) with bulk RNA-seq.

Methods: We identified M2-associated genes by intersecting TAM marker genes derived from scRNA-seq with macrophage module genes from WGCNA RNA-seq data. Prognostic M2 TAM-related genes were determined using univariate Cox and LASSO regression analyses. In the following steps, prognostic characteristics, risk groups, and external validation were constructed and validated. The immune landscape of patients with GBM was examined by evaluating immune cells, functions, evasion scores, and checkpoint genes.

Results: Analysis of scRNA-seq and bulk-seq data revealed 107 genes linked to M2 TAMs. Using univariate Cox and LASSO regression, 16 genes were identified as prognostic for GBM, leading to the creation and validation of a prognostic signature for GBM survival prediction.

Conclusion: Our findings reveal the immune landscape of GBM and enhance understanding of the molecular mechanisms associated with M2 TAMs.

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胶质母细胞瘤中与M2肿瘤相关巨噬细胞浸润相关的生物标志物鉴定。

目的：M2表型肿瘤相关巨噬细胞（tumor-associated macrophages, tam）可促进肿瘤生长、侵袭、耐化疗等，导致恶性进展。本研究的目的是通过整合单细胞RNA测序（scRNA-seq）和大量RNA-seq来鉴定胶质母细胞瘤（GBM）的新预后特征。方法：我们通过将来自scRNA-seq的TAM标记基因与来自WGCNA RNA-seq数据的巨噬细胞模块基因交叉鉴定m2相关基因。使用单变量Cox和LASSO回归分析确定预后M2 tam相关基因。在接下来的步骤中，构建和验证预后特征、风险组和外部验证。通过评估免疫细胞、功能、逃避评分和检查点基因来检查GBM患者的免疫景观。结果：scRNA-seq和bulk-seq数据分析显示107个与M2 tam相关的基因。使用单变量Cox和LASSO回归，16个基因被确定为GBM的预后，从而创建和验证了GBM生存预测的预后特征。结论：我们的研究结果揭示了GBM的免疫景观，并加强了对M2 tam相关分子机制的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Neurology CLINICAL NEUROLOGYNEUROSCIENCES -NEUROSCIENCES

CiteScore

4.90

自引率

8.80%

发文量

2792

审稿时长

14 weeks

期刊介绍： The section Stroke aims to quickly and accurately publish important experimental, translational and clinical studies, and reviews that contribute to the knowledge of stroke, its causes, manifestations, diagnosis, and management.