The short-term effect of nitrogen and phosphorus fertilizers on cold resistance in Urtica cannabina based on transcriptomics and metabolomics analysis.
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引用次数: 0
Abstract
Introduction: Freezing injury in winter is a major abiotic stress that significantly limits plant growth and survival. While nitrogen and phosphorus fertilizers have been demonstrated to alleviate the impact of freezing injury in various plant species, their role of fertilizers in the cold tolerance of Urtica spp. is still unknown.
Methods: This study investigated the effects of fertilizers on the cold resistance of U. cannabina by comprehensively analyzing the physiological and biochemical indices, transcriptome, and metabolome of the U. cannabina under applications of 150 kg nitrogen ha-1 (N) and 90 kg phosphorus ha-1 (P), using "no fertilizer" (CK) as the control.
Results: The results showed that applying nitrogen and phosphorus fertilizers reduced the malondialdehyde concentration and had much higher superoxide dismutase activity and soluble sugar and proline concentrations. Transcriptomics and metabolomics analysis revealed that applying nitrogen and phosphorus fertilizers tended to involve several critical regulatory pathways in the biosynthesis of secondary metabolites, flavonoid biosynthesis, and phenylpropanoid biosynthesis pathways. Concretely speaking, these fertilizers can affect the biosynthesis of naringenin, pinobanksin 3-acetate, galangin, and p-Coumaroyl shikimic acid and the expression of related genes to regulate the cold tolerance of U. cannabina. Moreover, through using weighted correlation network analysis (WGCNA), 4210 genes in response to nitrogen fertilizer and 5975 genes in response to phosphorus fertilizer, positively correlating with key metabolites, were identified. Several genes encoding enzymes including glucan endo-1,3-beta-glucosidase, pectinesterase, trehalase, hydroquinone glucosyltransferase, monodehydroascorbate reductase, tyrosine aminotransferase, and peroxidase were verified to be hub genes involved in the cold-stress response of U. cannabina.
Discussion: Overall, these findings have laid a theoretical foundation for the highly efficient utilization of nitrogen and phosphorus in U. cannabina and provide novel insights into the regulatory network of U. cannabina in response to cold-temperature stress.
摘要冬季冻害是严重限制植物生长和存活的主要非生物胁迫。虽然氮肥和磷肥已被证明可以减轻各种植物的冻害影响，但它们在荨麻属植物耐冷性中的作用尚不清楚。方法：以“不施肥”（CK）为对照，综合分析在150 kg hm -1氮(N)和90 kg hm -1磷(P)处理下大麻大麻的生理生化指标、转录组和代谢组学，研究施肥对大麻大麻抗寒性的影响。结果：施用氮肥和磷肥降低了植株丙二醛浓度，提高了植株超氧化物歧化酶活性、可溶性糖和脯氨酸浓度。转录组学和代谢组学分析表明，施用氮肥和磷肥往往涉及次生代谢物生物合成、类黄酮生物合成和苯丙类生物合成的几个关键调控途径。具体来说，这些肥料可以影响柚皮素、3-乙酸木皂苷、高良姜素和对香豆素酸的生物合成以及相关基因的表达，从而调控大麻素的耐寒性。此外，通过加权相关网络分析（WGCNA），鉴定出4210个氮肥响应基因和5975个磷肥响应基因与关键代谢物正相关。几个编码葡聚糖内切-1,3- β -葡萄糖苷酶、果胶酯酶、海藻化酶、对苯二酚葡萄糖基转移酶、单脱氢抗坏血酸还原酶、酪氨酸氨基转移酶和过氧化物酶的基因被证实是参与大麻冷胁迫反应的枢纽基因。综上所述，这些发现为大麻大麻高效利用氮和磷奠定了理论基础，并为大麻大麻应对低温胁迫的调控网络提供了新的见解。
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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