Salt-induced Reduction of Hyperswarming Motility in Bacillus cereus MHS is Associated with Reduction in Flagellation, Nanotube Formation and Quorum Sensing Regulator plcR.

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Nirbhay K Bhadani, Kritika Prasad, Nisha Gupta, Hemanta Sarmah, Tapas K Sengupta
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Abstract

Bacteria have been known to thrive in challenging environmental niches through diverse phenomena. Swarming is one such favourable adaptations that could help bacteria survive extreme conditions. Therefore, targeting swarming is crucial for improving our understanding of bacterial motility and preventing related infections. Bacillus cereus, which causes food poisoning, has been shown to perform swarming, and salts like NaCl can act as a food preservative to control bacterial growth. To explore the possible alterations in the swarming of Bacillus cereus in the presence of salt, the present study encompasses the effect of NaCl on the swarming characteristics of a natural bacterial isolate, Bacillus cereus MHS, with a hyperswarming phenotype. Here we report that increased NaCl in growth media could induce a reduction in swarming motility and pattern of MHS on Luria agar plates. This observed reduction in swarming was found to be associated with reduced flagellation and a reduction in the abundance of bacterial nanotubes. Gene expression studies supported the phenotypic and ultrastructure observations as the expressions of bfla and ymdB genes, involved in formations of flagella and nanotubes, respectively, were found to be reduced in the swarming MHS cells in the presence of increased NaCl. It was also observed that the salt-induced reduction in swarming of MHS is associated with the reduced expression of the quorum sensing regulator gene plcR. This study first time reports the bacterial nanotubes in a Bacillus cereus strain indicating a possible link between the bacterial nanotube formation and hyperswarming phenotype in Bacillus cereus MHS.

盐诱导蜡样芽孢杆菌MHS的过热运动性降低与鞭毛、纳米管形成和群体感应调节因子plcR的减少有关。
众所周知,细菌通过各种现象在具有挑战性的环境中茁壮成长。蜂群就是这样一种有利的适应,可以帮助细菌在极端条件下生存。因此,瞄准蜂群对于提高我们对细菌运动的理解和预防相关感染至关重要。导致食物中毒的蜡样芽孢杆菌已经被证明可以进行蜂群繁殖,而像NaCl这样的盐可以作为食物防腐剂来控制细菌的生长。为了探索盐存在下蜡样芽孢杆菌群体的可能变化,本研究包括NaCl对具有过热表型的天然细菌分离物蜡样芽孢杆菌MHS群体特征的影响。在本研究中,我们报道了在生长培养基中增加NaCl会导致Luria琼脂板上MHS的群体运动和模式降低。这种观察到的蜂群减少被发现与鞭笞减少和细菌纳米管丰度减少有关。基因表达研究支持表型和超微结构观察,发现在NaCl增加的情况下,MHS细胞群中分别参与鞭毛和纳米管形成的bfla和ymdB基因的表达减少。还观察到,盐诱导的MHS群体减少与群体感应调节基因plcR的表达减少有关。本研究首次报道了蜡样芽孢杆菌菌株中的细菌纳米管,提示蜡样芽孢杆菌MHS中细菌纳米管的形成与过热表型之间可能存在联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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