Potential of bone marrow mesenchymal stem cells in iodine-induced autoimmune thyroiditis therapy.

IF 3.5 2区医学 Q2 ENDOCRINOLOGY & METABOLISM

European Thyroid Journal Pub Date : 2025-06-12 Print Date: 2025-06-01 DOI:10.1530/ETJ-24-0137

Xun Liu, Likun Cui, Jianwei Dong, Jiancong Ren, Dongdong Xu, Yanshuo Han, Jian Zhang

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引用次数: 0

Abstract

Objective: Hashimoto's thyroiditis (HT) is a prevalent autoimmune disease without a cure. Mesenchymal stem cells (MSCs) may offer the opportunity to improve autoimmune thyroiditis.

Methods: We replicated the pathogenic factors of HT and established a stable autoimmune thyroiditis model in NOD.H-2h4 mice by administering iodine for 12 weeks. We used orthotopic injection to transplant bone MSCs (BMSCs) into the thyroid. Immunohistochemistry, enzyme-linked immunosorbent assay, flow cytometry, and hematoxylin and eosin and immunofluorescence staining were used to evaluate the effects of cell transplantation.

Results: Orthotopic BMSC transplantation decreased serum thyroglobulin antibody and caspase 3 levels; increased proliferating cell nuclear antigen levels; decreased CD4+/CD3+ T cells, Th1/Th2, and Th17/Treg ratios; decreased TNF-alpha (a proinflammatory cytokine) and interferon-gamma levels; and increased transforming growth factor-beta and interleukin-10 levels. In addition, it increased CD90/S100A4 and CD90/TTF-1 co-expression.

Conclusion: Orthotopic BMSC transplantation improved the inflammatory environment by regulating the secretion of anti-inflammatory cytokines, promoting regeneration, and reducing apoptosis in the thyroid tissue. Bone marrow-derived stem cells inhibited T cell activation, maintained a balance between T cell subpopulation ratios, and halted thyroiditis progression. Finally, transplanted BMSCs could transform into fibroblasts and thyroid cells. This study elucidated the pathogenesis of HT and provided evidence supporting the potential of MSCs in HT treatments.

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骨髓间充质干细胞在碘诱导自身免疫性甲状腺炎治疗中的潜力

目的：桥本甲状腺炎（HT）是一种常见的自身免疫性疾病，无法治愈。间充质干细胞（MSC）可能提供改善自身免疫性甲状腺炎的机会。方法：复制HT致病因子，通过给药12周建立NOD.H-2h4小鼠自身免疫性甲状腺炎模型。我们采用原位注射将骨间充质干细胞（BMSCs）移植到甲状腺。采用免疫组织化学、酶联免疫吸附法、流式细胞术、血红素和伊红及免疫荧光染色评价细胞移植的效果。结果：原位骨髓间充质干细胞移植降低血清甲状腺球蛋白抗体和caspase 3水平；增殖细胞核抗原水平增高；CD4+/CD3+ T细胞、Th1/Th2和Th17/Treg比值降低；tnf - α（一种促炎细胞因子）和干扰素- γ水平降低；转化生长因子- β和白细胞介素-10水平增加。此外，CD90/S100A4和CD90/TTF-1的共表达增加。结论：原位骨髓间充质干细胞移植通过调节甲状腺组织中抗炎细胞因子的分泌，促进再生，减少细胞凋亡，改善了炎症环境。骨髓干细胞抑制T细胞活化，维持T细胞亚群比例的平衡，并阻止甲状腺炎的进展。最后，移植骨髓间充质干细胞可转化为成纤维细胞和甲状腺细胞。本研究阐明了HT的发病机制，并为MSCs在HT治疗中的潜力提供了证据。

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来源期刊

European Thyroid Journal Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

6.70

自引率

2.10%

发文量

156

期刊介绍： The ''European Thyroid Journal'' publishes papers reporting original research in basic, translational and clinical thyroidology. Original contributions cover all aspects of the field, from molecular and cellular biology to immunology and biochemistry, from physiology to pathology, and from pediatric to adult thyroid diseases with a special focus on thyroid cancer. Readers also benefit from reviews by noted experts, which highlight especially active areas of current research. The journal will further publish formal guidelines in the field, produced and endorsed by the European Thyroid Association.