Effects of different frequencies of electroacupuncture stimulation on the electroencephalogram and intracranial levels of prostaglandin D₂ and adenosine in rats under propofol anesthesia.

IF 2.4 3区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Acupuncture in Medicine Pub Date : 2025-06-01 Epub Date: 2025-05-28 DOI:10.1177/09645284251344329

Qing Chai, Jin Ma, Jinjun Shu, Huaiming Wang, Yiquan Xu, Shuying Zhu, Hongwei Zhang, Liangchun Yan, Jun Song, Bo Liu, Yiding Chen

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引用次数: 0

Abstract

Objective: To investigate the effects of different frequencies of electroacupuncture (EA) stimulation on the electroencephalogram (EEG) and intracranial levels of adenosine (Ad) and prostaglandin D₂ (PGD₂) in rats anesthetized using propofol.

Methods: Thirty-six rats were divided into the manual acupuncture (MA), high-frequency EA (HF-EA) and low-frequency EA (LF-EA) groups (n = 12/group). After intravenous anesthesia with propofol, an EEG was recorded before and after stimulation at bilateral ST36 and PC6 using 50-Hz stimulation (HF-EA group) or 2/15-Hz stimulation (LF-EA group) versus no electrical stimulation (MA group). The brains were collected after the experiment to determine cortical and subcortical Ad and PGD₂ levels. The ventrolateral preoptic nucleus (VLPO) was collected for Ad immunohistochemistry.

Results: There were changes in the δ wave in the LF-EA group and in the α, β and θ waves in the HF-EA group. Compared with the MA group, subcortical Ad levels were higher in the LF-EA group (0.96 ± 0.035 vs 0.93 ± 0.034 pmol/mL, p < 0.05). Cortical PGD₂ levels were higher in the LF-EA group than in the MA group (13.49 ± 0.440 vs 12.95 ± 0.436 pmol/mL, p < 0.05). VLPO Ad levels were significantly increased in the LF-EA group compared to both the HF-EA group (0.210 ± 0.0066 vs 0.201 ± 0.0065, p < 0.05) and the MA group (0.210 ± 0.0066 vs 0.199 ± 0.0095, p < 0.01).

Conclusion: LF-EA at bilateral ST36 and PC6 may increase the depth of anesthesia and sedation, possibly through increased intracranial PGD₂ and Ad levels. However, such an effect was not found with HF-EA.

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不同频率电针刺激对异丙酚麻醉大鼠脑电图及颅内前列腺素D2和腺苷水平的影响。

目的：探讨不同频率电针（EA）刺激对异丙酚麻醉大鼠脑电图及颅内腺苷（Ad）、前列腺素D2 （PGD2）水平的影响。方法：将36只大鼠随机分为手针刺组（MA）、高频电针组（HF-EA）和低频电针组（LF-EA），每组12只。异丙酚静脉麻醉后，分别用50 hz刺激（HF-EA组）或2/15 hz刺激（LF-EA组）和不用电刺激（MA组）分别记录双侧ST36和PC6刺激前后的脑电图。实验结束后采集大鼠大脑，测定皮质和皮质下Ad和PGD2水平。收集腹外侧视前核（VLPO）进行Ad免疫组化。结果：LF-EA组的δ波和HF-EA组的α、β、θ波均有改变。与MA组相比，LF-EA组皮质下Ad水平高于MA组（0.96±0.035 vs 0.93±0.034 pmol/mL）， LF-EA组的pg2水平高于MA组（13.49±0.440 vs 12.95±0.436 pmol/mL）。结论：LF-EA可能通过增加颅内PGD2和Ad水平，增加双侧ST36和PC6的麻醉和镇静深度。然而，在HF-EA中没有发现这种效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acupuncture in Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

4.70

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Acupuncture in Medicine aims to promote the scientific understanding of acupuncture and related treatments by publishing scientific investigations of their effectiveness and modes of action as well as articles on their use in health services and clinical practice. Acupuncture in Medicine uses the Western understanding of neurophysiology and anatomy to interpret the effects of acupuncture.