Flavobacterium algoriphilum sp. nov., Flavobacterium arabinosi sp. nov., Flavobacterium cryoconiti sp. nov., Flavobacterium galactosi sp. nov., Flavobacterium melibiosi sp. nov., and Flavobacterium algoris sp. nov., six novel cold-adapted bacteria isolated from glaciers.

IF 4 2区生物学 Q2 MICROBIOLOGY

BMC Microbiology Pub Date : 2025-05-28 DOI:10.1186/s12866-025-04067-4

Lei-Lei Yang, Yu-Hua Xin, Qing Liu

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引用次数: 0

Abstract

Background: Six novel cold-adapted bacteria, LB3P122^T, LT1R49^T, ZT3R17^T, ZT3R25^T, XS2P12^T, and GB2R13^T, were isolated from glaciers on the Tibetan Plateau. This study aimed to characterize their taxonomic status and elucidate their molecular adaptations to cold environments using a polyphasic approach.

Results: All strains were Gram-stain-negative, rod-shaped, and psychrophilic, growing at 0 °C with an optimum at 14-20 °C and at pH values of 6.0-8.0 (optimum pH 7.0). Analysis of the 16S rRNA gene sequences placed their taxonomic positions within the genus Flavobacterium, with similarities ranging from 97.2 to 98.4% to species with validly published names. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the six strains formed distinct clades with Flavobacterium gawalongense GSP16^T. Phylogenomic analysis showed that these strains clustered with Flavobacterium gawalongense GSP16^T and exhibited a close relationship with Flavobacterium urumqiense CGMCC 1.9230^T and Flavobacterium xinjiangense CGMCC 1.2749^T. Average nucleotide identity (ANI) values ranging from 82.5 to 93.6% and digital DNA-DNA hybridization (dDDH) values ranging from 26.1 to 51.5% between these strains and their closest relatives were well below the bacterial species delineation thresholds (95-96% ANI, 70% dDDH). The predominant fatty acids were iso-C_15:0 and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c). Genomic analysis identified genes associated with cryoprotection, oxidative stress response, cold-shock response, and osmoprotection in these strains, underscoring their adaptations to glacial environments.

Conclusions: Based on polyphasic taxonomic evidence, the strains represent six novel species within the genus Flavobacterium, with the proposed names Flavobacterium algoriphilum sp. nov. (LB3P122^T = CGMCC 1.11443^T = NBRC 114820^T), Flavobacterium arabinosi sp. nov. (LT1R49^T = CGMCC 1.11617^T = NBRC 114822^T), Flavobacterium cryoconiti sp. nov. (ZT3R17^T = CGMCC 1.11707^T = NBRC 114824^T), Flavobacterium galactosi sp. nov. (ZT3R25^T = CGMCC 1.11711^T = NBRC 114825^T), Flavobacterium melibiosi sp. nov. (XS2P12^T = CGMCC 1.23198^T = NBRC 114826^T), and Flavobacterium algoris sp. nov. (GB2R13^T = CGMCC 1.24741^T = NBRC 114830^T). These findings enhance our understanding of Flavobacterium diversity and cold adaptation in cryospheric ecosystems.

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从冰川中分离出的6种新型冷适应细菌：嗜藻黄杆菌、阿拉伯黄杆菌、低温黄杆菌、半乳黄杆菌、melibiosis黄杆菌和藻黄杆菌。

背景：从青藏高原冰川中分离到6种新型冷适应菌LB3P122T、LT1R49T、ZT3R17T、ZT3R25T、XS2P12T和GB2R13T。本研究旨在利用多相方法表征其分类地位，并阐明其对寒冷环境的分子适应。结果：所有菌株革兰氏染色阴性，呈棒状，嗜冷，在0℃条件下生长，最适生长温度为14 ~ 20℃，pH值为6.0 ~ 8.0（最适pH为7.0）。16S rRNA基因序列分析将其分类位置定位在黄杆菌属，与已发表的有效名称的物种相似度为97.2 ~ 98.4%。16S rRNA基因序列的系统发育分析表明，6株菌株与加瓦隆黄杆菌GSP16T形成了不同的分支。系统基因组分析表明，这些菌株与加瓦隆黄杆菌GSP16T聚类，与乌鲁木齐黄杆菌CGMCC 1.9230T和新疆黄杆菌CGMCC 1.2749T亲缘关系密切。这些菌株与其近亲的平均核苷酸一致性（ANI）值为82.5 ~ 93.6%，数字DNA-DNA杂交（dDDH）值为26.1 ~ 51.5%，远低于细菌种类划分阈值（95% ~ 96% ANI, 70% dDDH）。主要脂肪酸为iso-C15:0和特征3 （C16:1 ω7c和/或C16:1 ω6c）。基因组分析确定了这些菌株中与低温保护、氧化应激反应、冷休克反应和渗透保护相关的基因，强调了它们对冰川环境的适应。结论:根据多相分类学证据，这些菌株代表了黄杆菌属中的6个新种，分别命名为：黄杆菌属褐藻黄杆菌（LB3P122T = CGMCC 1.11443 T = NBRC 114820T）、阿拉伯黄杆菌属（LT1R49T = CGMCC 1.11617T = NBRC 114822T）、冷冻黄杆菌属（ZT3R17T = CGMCC 1.11707T = NBRC 114824T）、半乳黄杆菌属（ZT3R25T = CGMCC 1.11711T = NBRC 114825T）、黄杆菌属（ZT3R25T = CGMCC 1.11711T = NBRC 114825T）。黄杆菌melibiosi sp. 11 （XS2P12T = CGMCC 1.23198T = NBRC 114826T）和黄杆菌algoris sp. 11 （GB2R13T = CGMCC 1.24741T = NBRC 114830T）。这些发现增强了我们对冰冻圈生态系统中黄杆菌多样性和冷适应的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Microbiology 生物-微生物学

CiteScore

7.20

自引率

0.00%

发文量

280

审稿时长

3 months

期刊介绍： BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.