Denaturation methods for reusable magnetic biosensors†

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-05-23 DOI:10.1039/D5LC00040H
Suhyeon Park, Songeun Kim, Shan X. Wang and Jung-Rok Lee
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Abstract

Nanoscale biosensors for sensitive DNA detection require advanced and precise fabrication techniques, which make them highly expensive and result in low yield rates. For such DNA biosensors, sensor regeneration is highly desirable. In this study, we investigated the effectiveness of various denaturants, including ultrapure water, urea solution, tris-ethylenediaminetetraacetic acid buffer, and dimethyl sulfoxide (DMSO), for the denaturation of target DNAs hybridized to probe DNAs on sensors. We used giant magnetoresistive (GMR) biosensors equipped with a temperature control unit in conjunction with magnetic nanoparticles. To examine the effect of DNA sequence on denaturation efficiency, 14 orthogonal DNA pairs were designed and tested. Furthermore, to maintain a consistent sensitivity in subsequent measurements, we evaluated the integrity of the probe DNAs on the sensors after denaturation. Among all the denaturants tested, 40% DMSO demonstrated excellent performance in the denaturation of probe DNAs covalently bonded to the sensors, without any heating process. This optimal denaturant can be applied to other planar DNA biosensor systems; moreover, GMR biosensors can facilitate the evaluation of newly developed denaturants.

Abstract Image

可重复使用磁性生物传感器的变性方法。
用于敏感DNA检测的纳米级生物传感器需要先进和精确的制造技术,这使得它们非常昂贵且产率低。对于这样的DNA生物传感器,传感器再生是非常可取的。在这项研究中,我们研究了各种变性剂,包括超纯水、尿素溶液、三乙二胺四乙酸缓冲液和二甲基亚砜(DMSO),对杂交到传感器上的探针dna的目标dna变性的有效性。我们使用了巨磁阻(GMR)生物传感器,该传感器配备了温度控制单元,并与磁性纳米颗粒结合使用。为了考察DNA序列对变性效率的影响,设计并测试了14对正交DNA对。此外,为了在随后的测量中保持一致的灵敏度,我们评估了变性后传感器上探针dna的完整性。在所有测试的变性剂中,40% DMSO在不经过任何加热过程的情况下,对与传感器共价结合的探针dna变性表现优异。该最佳变性剂可应用于其他平面DNA生物传感器系统;此外,GMR生物传感器可以促进新开发的变性剂的评价。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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