A Simple and Highly Sensitive Mitochondrial Targeting Fluorescent Probe and Its Applications for the Detection of Intracellular Viscosity

IF 3 4区化学 Q2 CHEMISTRY, ANALYTICAL

Luminescence Pub Date : 2025-05-29 DOI:10.1002/bio.70205

Tingting Liang, Rui Guo, Meijie Xu, Kexin Hu, Weiying Lin

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引用次数: 0

Abstract

Viscosity is an important intracellular parameter which plays a significant role in cellular activities. Abnormal changes in viscosity could lead to inflammation and various types of diseases. Therefore, testing cellular microviscosity changes could help to develop new tools for disease diagnosis and treatment. To accurately monitor mitochondrial viscosity changes, a novel fluorescent probe called DWL-IY was developed by using the twisted intramolecular charge transfer (TICT) mechanism. The probe DWL-IY demonstrated high selectivity for viscosity and good photostability under both light and light-avoidance conditions. It also showed high sensitivity, with the fluorescence intensity increasing by 146-fold from methanol to glycerol and a large Stokes shift of 77 nm in methanol and 85 nm in glycerol. The positively charged DWL-IY molecule could be accurately colocalized in mitochondria and safely applied to Hela cells with low toxicity, responding sensitively to their mitochondrial viscosity variations.

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一种简单、高灵敏度的线粒体靶向荧光探针及其在细胞内黏度检测中的应用

黏度是一个重要的胞内参数，在细胞活动中起着重要作用。粘稠度的异常变化可能导致炎症和各种疾病。因此，检测细胞微粘度变化有助于开发疾病诊断和治疗的新工具。为了准确监测线粒体粘度变化，利用分子内电荷转移（TICT）机制，研制了一种新型荧光探针DWL-IY。该探针在光避光条件下均表现出较高的粘度选择性和良好的光稳定性。它也显示出很高的灵敏度，从甲醇到甘油的荧光强度增加了146倍，甲醇和甘油的Stokes位移分别为77 nm和85 nm。带正电的DWL-IY分子可以准确地定位在线粒体中，并安全地应用于Hela细胞，具有低毒性，对线粒体粘度变化敏感。

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来源期刊

Luminescence 生物-生化与分子生物学

CiteScore

5.10

自引率

13.80%

发文量

248

审稿时长

3.5 months

期刊介绍： Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.