Exploration of Different Pre-Processing and Drying Methods on Volatile Organic Compounds in Bletilla striata Based on GC-IMS

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-05-29 DOI:10.1002/rcm.10082

Rui Xu, Shanshan Wang, Yiyuan Luo, Ping Pan, Yitian Zhao, Juan Wang, Hongjiang Chen

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引用次数: 0

Abstract

Rationale

Bletilla striata (Thunb.) Rchb.f. (Orchidaceae), a traditional Chinese medicine herb known as baiji, exhibits diverse pharmacological activities. Despite extensive research on its phytochemistry, the volatile organic compounds (VOCs) of B. striata remain largely unexplored.

Methods

Gas chromatography-ion mobility spectrometry (GC-IMS) was used to analyze and compare the VOCs of three pre-processing methods (fresh, boiling, and steaming) and four drying methods (sun-drying [SD], vacuum freeze drying [VFD], hot air drying [HAD], microwave drying [MD]) in B. striata.

Results

A total of 137 VOCs were detected and 124 VOCs were identified, primarily including aldehydes, alcohols, and ketones. Principal component analysis (PCA) and fingerprint similarity analysis revealed that the drying method has a greater impact on VOCs than pre-processing method. Partial least squares-discriminant analysis (PLS-DA) and heat map cluster analysis identified 34 VOCs as main characteristic markers to differentiate the samples of B. striata with different processing methods. Additionally, the samples subjected to sequential steaming followed by sun-drying demonstrated a superior ability to retain both the quantity and variety of non-harmful VOC.

Conclusion

This research conducted a detailed analysis of the fundamental VOCs in B. striata and provided a scientific theoretical guidance for optimizing processing of B. striata medicinal materials.

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基于GC-IMS的白芨挥发性有机物不同预处理和干燥方法的探索

白芨的基本原理Rchb.f。（兰科），一种被称为白暨豚的传统中药，具有多种药理活性。尽管对其植物化学成分进行了广泛的研究，但对其挥发性有机化合物（VOCs）的研究仍然很少。方法采用气相色谱-离子迁移谱法（GC-IMS）对三种预处理方法（鲜、煮、蒸）和四种干燥方法（晒干[SD]、真空冷冻干燥[VFD]、热风干燥[HAD]、微波干燥[MD]）进行挥发性有机化合物分析比较。结果共检测到137种挥发性有机化合物，鉴定出124种，主要是醛类、醇类和酮类。主成分分析（PCA）和指纹相似度分析表明，干燥方法对VOCs的影响大于预处理方法。偏最小二乘判别分析（PLS-DA）和热图聚类分析鉴定出34种挥发性有机化合物为主要特征标记物，可用于区分不同处理方法的白刺草样品。此外，经过连续蒸蒸和晒干的样品显示出更强的能力来保留非有害挥发性有机化合物的数量和种类。结论本研究对纹草中基本挥发性有机化合物进行了详细的分析，为纹草药材的优化加工提供了科学的理论指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.