Exploring Omega-3's impact on postnatal cerebral cortex development: A histological, immuno-histochemical, and biochemical study in a valproic acid rat model

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-05-24 DOI:10.1016/j.tice.2025.102989

Mohammed Ahmed Shehata , Alaa Fawzy Abdelaal , Noha Emad Abd El Fattah Eladawy , Nancy Husseiny Hassan

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引用次数: 0

Abstract

Introduction

The anti-epileptic medication Valproic acid (VPA) can damagedamage DNA and interfere with the mitochondria's capacity to metabolize energy. Consequently, VPA is a strong teratogen for the progeny of expectant mothers. OM3FA's capacity to preserve the cell membrane integrity of neural tissues improves cognitive function and Alzheimer's disease.

Aim of work

To determine postnatal neuroprotective impact of Omega-3 on cerebral cortex in Valproic acid-treated rat model.

Material and methods

Forty adult pregnant female albino rats were randomized into 4 groups equally: the control negative group (group I) which didn’t receive any medications, the Omega3 treated group (group II) which received Omega3 (144 mg/Kg/ day) orally once daily and the Valproic Acid treated group (group III) which received Valproic Acid oral solution (50 mg/Kg/day) once daily using gastric tubes and the Valproic Acid +Omega3 treated group received VPA(50 mg/Kg/day) and Omega3(144 mg/Kg/ day) once daily. Then the cerebral cortex of all offspring were processed at 7th and 21th day after birth to be evaluated biochemically, histologically, immunohistochemically, and morphometrically.

Results

Histologically and immunohistochemically, the group treated with Valproic Acid revealed remarkable degenerative and neuronal apoptotic changes of the cerebral cortex six layers. The group treated with Valproic Acid+Omega3 showed enhancement in the histological, and immunohistochemical alterations. As regards the biochemical (MDA &TAC) and morphometric results Valproic Acid+Omega3 showed statistically significant improvement in comparison with the Valproic Acid.

Conclusion

Valproic acid consumption during pregnancy documented to have neurotoxic impact on cerebral cortex structure of the rat offspring. Omega3 has a protective effect against Valproic acid neurotoxicity.

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探索Omega-3对产后大脑皮层发育的影响：丙戊酸大鼠模型的组织学、免疫组织化学和生化研究

抗癫痫药物丙戊酸（VPA）会破坏DNA并干扰线粒体代谢能量的能力。因此，VPA对孕妇的后代是一种强致畸剂。OM3FA保持神经组织细胞膜完整性的能力可改善认知功能和阿尔茨海默病。目的探讨Omega-3对丙戊酸处理大鼠出生后大脑皮层的神经保护作用。材料与方法将40只成年妊娠雌性白化大鼠随机分为4组：对照组阴性组（I组）不接受任何药物治疗，omega - 3治疗组（II组）口服omega - 3(144 mg/Kg/ d)，丙戊酸治疗组（III组）口服丙戊酸溶液（50 mg/Kg/d），丙戊酸+ omega - 3治疗组口服VPA（50 mg/Kg/d）和omega - 3（144 mg/Kg/ d）。然后在出生后第7天和第21天对所有子代的大脑皮层进行生化、组织学、免疫组织化学和形态计量学评价。结果丙戊酸组大鼠大脑皮层6层出现明显的退行性改变和神经元凋亡。丙戊酸+欧米伽3组在组织学和免疫组化方面表现出增强。在生化（MDA & TAC）和形态计量学结果方面，丙戊酸+Omega3与丙戊酸相比有统计学上的显著改善。结论妊娠期丙戊酸摄入对子代大鼠大脑皮层结构有神经毒性影响。欧米伽3对丙戊酸的神经毒性有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.