Patching-up of major influencing factors of Rhizophagus irregularis cultivation for industrial production of consistent spore density through cascade statistical optimization

Q1 Environmental Science

Bioresource Technology Reports Pub Date : 2025-05-26 DOI:10.1016/j.biteb.2025.102160

Jubair Al Rashid , Md Abuhena , Jingjing Wang , Md. Faisal Azim , Zhiyong Huang

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Abstract

Arbuscular mycorrhizal fungi (AMF) play a crucial role as a climate-change solution, aiding nutrient uptake, interplant communication, and stress mitigation. However, its industrial-scale production remains limited. This study aimed to optimize production of Rhizophagus irregularis (Apex-MYK-01) in carrot root organ cultures using a cascading approach to achieve consistent spore yields. The first phase identified ideal conditions: 2 × 2 cm inoculum size, 100 mL media, and using 90-day-old inoculum in box-shaped vessels. The second phase revealed four critical factors (p < 0.05) influencing output. Final optimization of those four parameters via central composite design pinpointed optimal values: 80-day incubation, 1.25 cm media height, 0.875 % sugar, and 2.75 mg KH₂PO₄. When tested at industrial scale, these conditions boosted spore production by 16.8 % (2213 ± 11 vs. lab-scale 1895 ± 15 spores/g) while maintaining high root colonization rates (82 ± 11 %). This cascading strategy demonstrates a viable path to standardized, large-scale AMF production.

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用级联统计优化方法补齐不规则根噬菌工业化生产中孢子密度一致栽培的主要影响因素

丛枝菌根真菌（AMF）在气候变化解决方案中发挥着至关重要的作用，有助于养分吸收、植物间交流和缓解胁迫。然而，其工业规模的生产仍然有限。本研究旨在利用级联方法优化胡萝卜根器官培养中不规则根噬菌（Apex-MYK-01）的生产，以实现一致的孢子产量。第一阶段确定了理想条件：接种量为2 × 2 cm，培养基为100 mL，接种于90天龄的盒状容器中。第二阶段揭示了四个关键因素(p <；0.05)影响产出。通过中心组合设计对这四个参数进行优化，确定了最佳值：80天孵育，1.25 cm培养基高度，0.875%糖，2.75 mg KH₂PO₄。当在工业规模上进行测试时，这些条件使孢子产量提高了16.8%(2213±11比实验室规模的1895±15孢子/g)，同时保持较高的根定植率（82±11%）。这种级联策略展示了标准化、大规模AMF生产的可行途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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