Synergistic enhancement of desferrioxamine and strontium-doped hydroxyapatite via coaxial electrostatic spinning on osteogenic differentiation

Abstract

Defective bone repair is a pressing issue in the field of bone regeneration due to donor shortages and the associated complications of bone grafting. Artificial bone repair materials offer more options and are attracting extensive attention from researchers. However, achieving efficient osteogenesis in clinical practice remains a challenge. Here, dual drug-releasing nanofibre membranes (NMs) for bone repair was fabricated by coaxial electrostatic spinning. Strontium-doped hydroxyapatite (SrHA) and desferrioxamine (DFO) were loaded into the shell and core of the NMs for slow and sustained release, respectively. Our results showed that the DFO/SrHA NMs significantly enhanced cell proliferation, angiogenesis and osteogenic differentiation of rat bone mesenchymal stem cells through the synergism of DFO and SrHA. This synergy results from the slow and sustained release of DFO and SrHA via NMs, which maintains the drug concentration in the effective range for a long time, ensuring long-term stable activation of the HIF-1 $\alpha$ and Wnt/$\beta$-catenin signalling pathways. Overall, the DFO/SrHA NMs provides a promising strategy for defective bone repair by enhancing osteogenic differentiation.