Evaluating the adjuvant potential of NK-lysin and HMGB1-derived peptides in enhancing immune responses in Salmo salar

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-05-27 DOI:10.1016/j.fsi.2025.110462

Crisleri Carrera , Milton F. Villegas , Carolina Muñoz-Flores , Leonardo Ortega , Santiago Salazar , Ariel Valenzuela , Niza Agurto , Raquel Montesino , Alex Romero , Bastian Couso , Jorge Saavedra , Rafael Maura , Natalie Parra , Fátima Reyes , Alfredo Saavedra , Oliberto Sánchez , Jorge R. Toledo , Jannel Acosta

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引用次数: 0

Abstract

The development of effective vaccines for aquaculture is crucial to prevent infectious diseases and improve fish health. Inactivated and subunit vaccines are commonly used in salmon farming; however, their immunogenicity is often limited, requiring adjuvants to enhance their efficacy. In this study, we evaluated the adjuvant potential of immunomodulatory peptides derived from HMGB1 (11W) and NK-lysin (NK1 and NK4) in formulations containing the VP2 antigen of infectious pancreatic necrosis virus (IPNv) in Salmo salar. The peptides were co-administered with the recombinant VP2 protein by intraperitoneal injection in different experimental groups, and serum samples were subsequently analyzed to determine IgM levels. Furthermore, the induction of the cytokines IFN-γ, IL-17, and IL-4/13A in tissues and leukocytes from head kidneys re-stimulated with the antigen was evaluated by RT-qPCR. The results indicated that the formulations containing NK1, NK4, and 11W progressively stimulated IgM production from day 35 post-vaccination. Furthermore, these adjuvant peptides significantly induced the expression of the evaluated cytokines in the head kidney and spleen of immunized animals and in the primary culture model, suggesting the activation of Th1, Th2, and Th17 responses. These findings suggest that peptide adjuvants can enhance the immune response in salmonid vaccination, offering a promising alternative to conventional adjuvants.

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评估nk -溶酶和hmgb1衍生肽增强Salmo salar免疫应答的佐剂潜力

开发有效的水产养殖疫苗对预防传染病和改善鱼类健康至关重要。灭活疫苗和亚单位疫苗通常用于鲑鱼养殖；然而，它们的免疫原性通常是有限的，需要佐剂来增强其功效。在这项研究中，我们评估了从HMGB1 （11W）和NK-lysin （NK1和NK4）衍生的免疫调节肽在含有感染性胰腺坏死病毒（IPNv） VP2抗原的配方中的佐剂潜力。在不同的实验组中，将这些肽与重组VP2蛋白共同腹腔注射，随后分析血清样本以测定IgM水平。此外，通过RT-qPCR评估抗原再刺激的头肾组织和白细胞中IFN-γ、IL-17和IL-4/13A细胞因子的诱导作用。结果表明，含有NK1、NK4和11W的配方从接种后第35天开始逐渐刺激IgM的产生。此外，这些佐剂肽显著诱导免疫动物头部肾脏和脾脏以及原代培养模型中所评估的细胞因子的表达，表明激活了Th1， Th2和Th17反应。这些发现表明，肽佐剂可以增强沙门氏菌疫苗接种的免疫反应，为传统佐剂提供了一个有希望的替代品。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.