Heightened effector immune cell infiltration of the hippocampus concurrently with brain ventricular volume expansion in aged APP/PS1 mice

IF 2.5 4区医学 Q3 IMMUNOLOGY

Journal of neuroimmunology Pub Date : 2025-05-26 DOI:10.1016/j.jneuroim.2025.578646

Mark A. Maynes , Carley A. Owens , Delaney M. Anani-Wolf , Zachariah P. Tritz , Fang Jin , Michael J. Hansen , Marina Seady , Aaron J. Johnson

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引用次数: 0

Abstract

Alzheimer's disease (AD) is the most common form of dementia for which the role of neuroinflammation is becoming more realized. Recent studies have shown that immune cells infiltrate the hippocampus and the cortex of AD patients as well as mouse models of the disease. In this study, we employed T2-weighted magnetic resonance imaging (MRI) to view changes in ventricular volume in addition to spectral flow cytometric assessment of the hippocampus infiltrating immune profile in the aged APP/PS1 mice. Aged APP/PS1 mice present with increased size of lateral, dorsal, and ventral ventricles plus increased numbers of hippocampus infiltrating effector immune cell subsets, including CD8 T cells expressing IFNγ, granzyme B, and perforin along with other cell types such as γδ T cells, neutrophils, and NK cells. The concurrent increase in effector cell types with ventricular enlargement expands the putative mechanism of brain atrophy in the APP/PS1 mouse model to include cytolytic functions of these aforementioned immune cell subsets.

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老年APP/PS1小鼠海马效应免疫细胞浸润增强，同时脑室体积增大

阿尔茨海默病（AD）是最常见的痴呆症形式，神经炎症的作用越来越被认识到。最近的研究表明，免疫细胞浸润阿尔茨海默病患者的海马和皮层以及该疾病的小鼠模型。在本研究中，我们采用t2加权磁共振成像（MRI）观察老年APP/PS1小鼠的心室容积变化，并采用流式细胞术评估海马浸润免疫谱。老年APP/PS1小鼠的侧脑室、背脑室和腹侧脑室的大小增加，海马浸润效应免疫细胞亚群的数量增加，包括表达IFNγ、颗粒酶B和穿孔素的CD8 T细胞，以及其他细胞类型，如γδ T细胞、中性粒细胞和NK细胞。在APP/PS1小鼠模型中，效应细胞类型的增加与脑室增大同时发生，扩展了脑萎缩的假设机制，包括上述免疫细胞亚群的细胞溶解功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of neuroimmunology 医学-免疫学

CiteScore

6.10

自引率

3.00%

发文量

154

审稿时长

37 days

期刊介绍： The Journal of Neuroimmunology affords a forum for the publication of works applying immunologic methodology to the furtherance of the neurological sciences. Studies on all branches of the neurosciences, particularly fundamental and applied neurobiology, neurology, neuropathology, neurochemistry, neurovirology, neuroendocrinology, neuromuscular research, neuropharmacology and psychology, which involve either immunologic methodology (e.g. immunocytochemistry) or fundamental immunology (e.g. antibody and lymphocyte assays), are considered for publication.