A highly sensitive biosensor for estradiol detection in environmental water samples using aptamer conformational switching and RCA-Cas12a amplification

IF 8.2 Q1 ENVIRONMENTAL SCIENCES
Chenqi Niu , Chong Zhang , Changwen Ye
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引用次数: 0

Abstract

Estradiol poses a potential threat to human health through bioaccumulation in aquatic food chains. The CRISPR-Cas system has revolutionized nucleic acid-based detection. However, adapting CRISPR-based diagnostics for small molecule detection remains a significant challenge. In this study, a biosensor for estradiol detection was developed using an aptamer-based strategy. This biosensor employs a high-affinity estradiol-binding aptamer (CN-Es2), which was previously identified by our group, to construct a “microbead-capture strand-blocker” functional probe. By integrating rolling circle amplification (RCA) with Cas12a-mediated nucleic acid cleavage, the system achieved robust signal amplification, enabling the highly sensitive and specific detection of estradiol. Moreover, this biosensor exhibited a dynamic detection range of 10 pM to 10 nM, with a detection limit of 19.2 pM (5.2 ng/L). Overall, this method provides a rapid and efficient strategy for monitoring trace levels of estradiol in complex matrices, addressing a critical requirement in the context of public food safety.
利用适体构象开关和RCA-Cas12a扩增技术,用于环境水样中雌二醇检测的高灵敏度生物传感器
雌二醇通过在水生食物链中的生物积累对人类健康构成潜在威胁。CRISPR-Cas系统彻底改变了基于核酸的检测。然而,将基于crispr的诊断方法用于小分子检测仍然是一个重大挑战。在本研究中,利用适配体为基础的策略开发了一种用于雌二醇检测的生物传感器。该生物传感器采用高亲和力雌二醇结合适配体(CN-Es2),这是我们的团队之前发现的,构建了一个“微珠捕获链阻断剂”功能探针。该系统将滚动环扩增(rolling circle amplification, RCA)与cas12a介导的核酸裂解相结合,实现了鲁棒的信号扩增,实现了雌二醇的高灵敏度和特异性检测。该传感器动态检测范围为10 pM ~ 10 nM,检出限为19.2 pM (5.2 ng/L)。总的来说,该方法为监测复杂基质中痕量雌二醇水平提供了一种快速有效的策略,解决了公共食品安全背景下的关键要求。
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来源期刊
CiteScore
15.40
自引率
0.00%
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