Integrated QTL mapping and transcriptomic profiling elucidate molecular determinants of sucrose accumulation in apricot (Prunus armeniaca L)

IF 5.4 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2025-05-23 DOI:10.1016/j.cpb.2025.100500

Fengchao Jiang , Li Yang , Junhuan Zhang , Meiling Zhang , Wenjian Yu , Haoyuan Sun

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引用次数: 0

Abstract

Apricot is a commercially vital stone fruit prized for its distinctive organoleptic characteristics and nutritional value. We developed an F1 population from ‘Chuanzhihong’ × ‘Luotuohuang’ to investigate the genetic basis of sugar metabolism. Whole-genome resequencing identified 63,162 high-quality InDel markers, while metabolomic profiling quantified soluble sugars (sucrose, glucose, fructose) across fruit developmental stages. Continuous variation in sugar accumulation patterns indicated polygenic inheritance. A high-density genetic map (601.5 cM, 0.44 cM average interval) revealed 20 stable QTLs across 8 linkage groups, explaining 8.0–16.2 % phenotypic variance for individual and total sugars. WGCNA identified sugar component-specific modules showing strong correlations (r = 0.57–0.95). Integrative analysis prioritized PA08G27233 as a hub gene within QTL intervals, encoding a SWEET transporter (designated PaSWEET1) with evolutionary conservation to AtSWEET1. This study elucidates molecular mechanisms of photoassimilate partitioning in apricot and provides genomic resources (high-resolution map, candidate genes) for marker-assisted breeding. The findings advance functional characterization of sugar metabolism pathways in Prunus species.

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综合QTL定位和转录组学分析阐明杏（Prunus armeniaca L）蔗糖积累的分子决定因素

杏是一种商业上重要的核果，因其独特的感官特征和营养价值而受到珍视。为研究糖代谢的遗传基础，以‘川致红’× ‘骆驼黄’为材料，选育了一个F1群体。全基因组重测序鉴定出63,162个高质量InDel标记，代谢组学分析量化了果实发育阶段的可溶性糖（蔗糖、葡萄糖、果糖）。糖积累模式的持续变化表明多基因遗传。高密度遗传图谱（601.5 cM， 0.44 cM平均间隔）揭示了8个连锁群中的20个稳定qtl，解释了个体糖和总糖的8.0 - 16.2% %表型变异。WGCNA鉴定出糖组分特异性模块具有很强的相关性（r = 0.57-0.95）。整合分析将PA08G27233作为QTL区间内的枢纽基因，编码一个SWEET转运体（命名为PaSWEET1），并具有进化保守性。本研究阐明了杏光同化物质分配的分子机制，为标记辅助育种提供了基因组资源（高分辨率图谱、候选基因）。研究结果为李属植物糖代谢途径的功能表征提供了新的思路。

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来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.