Optimization of laser induced periodic surface structures for surface enhanced Raman spectroscopy of extracellular vesicles

IF 2.2 3区物理与天体物理 Q2 OPTICS

Optics Communications Pub Date : 2025-05-15 DOI:10.1016/j.optcom.2025.131952

Simon Barter , Genevieve Boom , Jeffery Low , Francesco Merola , Claude Aguergaray , Larry Chamley , Lynsey Cree , Neil G.R. Broderick

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引用次数: 0

Abstract

Gold coated laser-induced periodic surface structures (LIPSS) on stainless steel are proposed as effective substrates for surface-enhanced Raman spectroscopy (SERS). The nano-structuring of steel wafers by fem- tosecond laser pulses is achieved and optimized for use in analysing extracellular vesicles (EVs). A variety of parameters are considered – such as writing laser power, polarization, machining speed, and gold layer thick- ness – each with implications for the LIPSS morphology and, in turn, the SERS enhancement. Ultimately, the optimized nanostructure is shown to contribute an in- crease in the SERS enhancement factor of one order of magnitude. As the LIPSS are fabricated via a ’one- step’ method, the proposed device reduces both cost and manufacturing time compared to industry standard techniques. The capability of machined structures in detecting extracellular vesicles (EVs) from a choriocar- cinoma cell line (JEG-3), commonly used as a model for placental syncytiotrophoblasts, is demonstrated and applications in the detection of different diseases are discussed.

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细胞外囊泡表面增强拉曼光谱的激光诱导周期表面结构优化

提出了在不锈钢表面镀金激光诱导周期性表面结构（LIPSS）作为表面增强拉曼光谱（SERS）的有效衬底。利用有限元-秒激光脉冲实现了钢晶片的纳米结构，并对其进行了优化，以用于分析细胞外囊泡（ev）。考虑了各种参数-例如写入激光功率，偏振，加工速度和金层厚度-每个参数都影响LIPSS的形态，进而影响SERS增强。结果表明，优化后的纳米结构使SERS增强因子提高了一个数量级。由于LIPSS是通过“一步”方法制造的，与工业标准技术相比，所提出的设备降低了成本和制造时间。本文展示了机械结构检测绒毛膜癌细胞系（JEG-3）细胞外囊泡（ev）的能力，并讨论了其在检测不同疾病中的应用。

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来源期刊

Optics Communications 物理-光学

CiteScore

5.10

自引率

8.30%

发文量

681

审稿时长

38 days

期刊介绍： Optics Communications invites original and timely contributions containing new results in various fields of optics and photonics. The journal considers theoretical and experimental research in areas ranging from the fundamental properties of light to technological applications. Topics covered include classical and quantum optics, optical physics and light-matter interactions, lasers, imaging, guided-wave optics and optical information processing. Manuscripts should offer clear evidence of novelty and significance. Papers concentrating on mathematical and computational issues, with limited connection to optics, are not suitable for publication in the Journal. Similarly, small technical advances, or papers concerned only with engineering applications or issues of materials science fall outside the journal scope.