Hyperoside improves intestinal mucosal immunity against zearalenone-induced intestinal barrier damage by regulating intestinal flora

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2025-05-20 DOI:10.1016/j.vetimm.2025.110949

Tianyu Han , Yan Jiang , Zhijun Liu , Lulu Wang , Yiding Liu , Shanshan Fei , Yu Yang , Tong Wang , Baiwen Guan , Mengran Cui , Qi Zhang , Haibin Wang , Guangliang Shi

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引用次数: 0

Abstract

Zearalenone (ZEA) is a mycotoxin that is immunotoxic and causes intestinal damage. Hyperoside (HYP) is a natural flavonol side with a wide range of sources and has a variety of pharmacological effects. The aim of this study is to investigate the effect and mechanism of HYP on ΖΕΑ-induced intestinal immunosuppression and intestinal injury in piglets. Histological and ultrastructural changes in the ileum of piglets were observed by H&E staining and transmission electron microscopy. The changes of intestinal microorganisms in the ileum of piglets were detected by 16S rRNA technology. Intestinal chemical barriers (MUC-1 and MUC-2), and physical barriers (β-Catenin, TJ-3, TJ-2, MYLK, Claudin2, and Claudin3) were measured by qRT-PCR. The intestinal immune barrier (sIg A) was detected by Elisa. Immune-related cytokines (TLR-4, IL-1β, IFN-γ, IL-18, IL-6, IL-17, IL-8, IL-25, and TNF-α) were detected by qRT-PCR. The content of ZEA in serum and ileum tissue was detected by Elisa. WB and qRT-PCR were used to detect ferroptosis related indicators (SLC7A11, Gpx4, FTH1, PTGS2, and ACSL4). Our results showed that HYP attenuated ZEA-induced tissue and ultrastructure damage and restored the richness and diversity of intestinal flora in the ileum of piglets. In addition, HYP also alleviated the accumulation of ZEA in the intestine and serum by restoring the chemical, physical and immunological barriers of the ileum. Moreover, HYP was found to attenuate ZEA-induced intestinal ferroptosis. Taken together, our study suggests that HYP can be used as an effective strategy to mitigate ZEA exposure-induced intestinal barrier damage and immune suppression in piglets.

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金丝桃苷通过调节肠道菌群，提高肠道黏膜免疫力，抵抗玉米赤霉烯酮诱导的肠道屏障损伤

玉米赤霉烯酮（ZEA）是一种具有免疫毒性的真菌毒素，可引起肠道损伤。金丝桃苷（Hyperoside， HYP）是一种来源广泛的天然黄酮醇类物质，具有多种药理作用。本研究旨在探讨HYP对仔猪ΖΕΑ-induced肠道免疫抑制和肠道损伤的影响及其机制。采用H&；E染色和透射电镜观察仔猪回肠的组织学和超微结构变化。采用16S rRNA技术检测仔猪回肠内肠道微生物的变化。采用qRT-PCR检测肠道化学屏障（MUC-1和MUC-2）和物理屏障（β-Catenin、TJ-3、TJ-2、MYLK、Claudin2和Claudin3）。Elisa法检测小鼠肠道免疫屏障（sIg A）水平。qRT-PCR检测免疫相关细胞因子（TLR-4、IL-1β、IFN-γ、IL-18、IL-6、IL-17、IL-8、IL-25、TNF-α）。Elisa法检测大鼠血清和回肠组织中ZEA的含量。WB和qRT-PCR检测铁下垂相关指标（SLC7A11、Gpx4、FTH1、PTGS2、ACSL4）。结果表明，HYP可减轻zea诱导的仔猪组织和超微结构损伤，恢复仔猪回肠菌群的丰富度和多样性。此外，HYP还通过恢复回肠的化学、物理和免疫屏障，减轻了ZEA在肠道和血清中的积累。此外，发现HYP可减轻zea诱导的肠铁下垂。综上所述，本研究表明，HYP可作为减轻ZEA暴露引起的仔猪肠道屏障损伤和免疫抑制的有效策略。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.