ZnO@CuS Nanoprobe-Assisted Electrochemical Biosensor Based on Multiple Cyclic Amplification for Highly Sensitive Detection of MicroRNA-155

IF 5.5 2区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Applied Nano Materials Pub Date : 2025-05-15 DOI:10.1021/acsanm.5c0168810.1021/acsanm.5c01688

Yanci Zhang, Yujin Fan, Jiyuan Zhang, Yanxi Hu, Lili Duan, Zhanhui Wang, Yihong Wang, Jiansheng Cui* and Liang Tian*,

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引用次数: 0

Abstract

The imbalanced expression of microRNA is related to many diseases and tumors, which can be used as a noninvasive biomarker to assist in the diagnosis, prognosis, and monitoring of cancer. A target conversion strategy-based electrochemical biosensor was developed by DNAzyme-cleavage dual cyclic signal amplification and the strand displacement reaction (SDR) to produce high electrochemical signals for microRNA-155 (miRNA-155) analysis. Thereinto, VSe₂/multi-walled carbon nanotubes (MWCNTs) with high conductivity were applied as the electrode surface material to provide more active sites and accelerate electron transportation. Then, the excellent immobilization efficiency and large specific surface area of the ZnO@CuS nanoprobes were applied as a recognition molecule carrier. Furthermore, hexaammineruthenium(III) chloride (RuHex), with superior electrochemical redox activity, acted as the electroactive indicator by intercalating into double-helix DNA through electrostatic interaction, which produced high current responses in the presence of the target. Therefore, this miRNA-155 biosensing platform exhibited a relatively low limit of detection of 0.11 fM, ranging from 0.5 fM to 5 nM, and demonstrated excellent repeatability and stability. Hence, this platform appeared to possess favorable human serum detection capacity and be suitable for early cancer diagnostics.

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ZnO@CuS基于多次循环扩增的纳米探针辅助电化学生物传感器对MicroRNA-155的高灵敏度检测

microRNA的不平衡表达与许多疾病和肿瘤有关，可以作为一种无创生物标志物，辅助癌症的诊断、预后和监测。利用DNAzyme-cleavage双循环信号放大和链位移反应（SDR），研制了一种基于靶标转换策略的电化学生物传感器，产生高电化学信号用于分析microRNA-155 （miRNA-155）。其中，采用高电导率的VSe2/多壁碳纳米管（MWCNTs）作为电极表面材料，提供更多的活性位点，加速电子传递。然后，利用ZnO@CuS纳米探针优异的固定化效率和较大的比表面积作为识别分子载体。此外，具有优异电化学氧化还原活性的六胺矿氯化钌（RuHex）通过静电相互作用嵌入到双螺旋DNA中，作为电活性指示剂，在目标物存在时产生高电流响应。因此，该miRNA-155生物传感平台的检测限较低，为0.11 fM，范围为0.5 fM ~ 5 nM，具有良好的重复性和稳定性。因此，该平台具有良好的人血清检测能力，适用于早期癌症诊断。

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来源期刊

ACS Applied Nano Materials Multiple-

CiteScore

8.30

自引率

3.40%

发文量

1601

期刊介绍： ACS Applied Nano Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics and biology relevant to applications of nanomaterials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important applications of nanomaterials.