Capillary Force-Driven Capture of Magnetic Nanoparticles in Calcium Phosphate Hollow-Tube Whisker Scaffolds for Osteonecrosis of the Femoral Head

IF 15.8 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

ACS Nano Pub Date : 2025-05-28 DOI:10.1021/acsnano.5c02874

Yi Zhou, Cong Feng, Xiaolong Yang, Jiang Yu, Xiangfeng Li, Weili Fu, Xiangdong Zhu, Jian Li, Xingdong Zhang

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Abstract

Excessive glucocorticoid use disrupts osteogenesis and angiogenesis in the femoral head, leading to steroid-induced osteonecrosis of the femoral head (SONFH), which is a significant clinical challenge. This study introduces a magnetically responsive biphasic calcium phosphate (HBCP/Fe₃O₄) scaffold featuring a nanoparticle-embedded hollow-tube whisker structure. The scaffold was fabricated through an in situ growth process to generate hollow-tube whiskers, followed by a capillary trapping technique that allowed the hollow-tube whiskers to capture Fe₃O₄ nanoparticles (NPs), achieving uniform and efficient encapsulation. HBCP/Fe₃O₄ exhibited excellent magnetic responsiveness and significant biological effects under static magnetic field (SMF) stimulation. In vitro, HBCP/Fe₃O₄ under SMF promoted osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in a glucocorticoid microenvironment, enhanced angiogenesis in human umbilical vein endothelial cells (HUVECs), and induced M2 polarization of RAW 264.7 murine macrophage cells (RAW 264.7). Furthermore, HBCP/Fe₃O₄ under SMF stimulation orchestrated paracrine signaling from endothelial and immune cells, thereby enhancing the osteogenic differentiation of BMSCs. Mechanistically, the osteogenic differentiation of BMSCs was driven by magnetic stimulation-induced Piezo1-mediated Ca²⁺ influx, which activated BMP-2/Smad signaling and upregulated key osteogenic markers. In vivo, the implantation of HBCP/Fe₃O₄ scaffolds under SMF stimulation in a rabbit SONFH model promoted coordinated therapeutic effects, including robust bone regeneration, in situ revascularization, immunomodulation, and preservation of femoral head cartilage. Together, these findings support the clinical relevance of this magnetically responsive scaffold as a multifunctional strategy for delaying structural deterioration and facilitating comprehensive repair in SONFH.

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毛细管力驱动磁性纳米颗粒在磷酸钙空心管晶须支架中的捕获用于股骨头坏死

过量使用糖皮质激素会破坏股骨头的成骨和血管生成，导致类固醇性股骨头骨坏死（SONFH），这是一个重大的临床挑战。本研究介绍了一种磁响应的双相磷酸钙（HBCP/Fe3O4）支架，具有纳米颗粒嵌入的空心管晶须结构。该支架通过原位生长过程生成空心管晶须，然后通过毛细管捕获技术使空心管晶须捕获Fe3O4纳米颗粒（NPs），从而实现均匀有效的封装。HBCP/Fe3O4在静磁场（SMF）刺激下表现出优异的磁响应性和显著的生物效应。体外，SMF下HBCP/Fe3O4促进糖皮质激素微环境下骨髓间充质干细胞（BMSCs）的成骨分化，增强人脐静脉内皮细胞（HUVECs）的血管生成，诱导小鼠巨噬细胞（RAW 264.7） M2极化。此外，HBCP/Fe3O4在SMF刺激下协调内皮细胞和免疫细胞的旁分泌信号，从而增强骨髓间充质干细胞的成骨分化。在机制上，骨髓间充质干细胞的成骨分化是由磁刺激诱导的piezo1介导的Ca2+内流驱动的，这激活了BMP-2/Smad信号并上调了关键的成骨标志物。在体内，HBCP/Fe3O4支架在SMF刺激下植入兔SONFH模型，促进了协调的治疗效果，包括强健的骨再生、原位血运重建、免疫调节和股骨头软骨的保存。总之，这些发现支持了这种磁响应支架作为延迟SONFH结构恶化和促进全面修复的多功能策略的临床相关性。

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来源期刊

ACS Nano 工程技术-材料科学：综合

CiteScore

26.00

自引率

4.10%

发文量

1627

审稿时长

1.7 months

期刊介绍： ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.