Thiocapsa, Lutimaribacter, and Delftia Are Major Bacterial Taxa Facilitating the Coupling of Sulfur Oxidation and Nutrient Recycling in the Sulfide-Rich Isinuka Spring in South Africa.

IF 3.6 3区 生物学 Q1 BIOLOGY
Henry Joseph Oduor Ogola, Ramganesh Selvarajan, Somandla Ncube, Lawrence Madikizela
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Abstract

Sulfur cycling is a fundamental biogeochemical process, yet its microbial underpinnings in environments like the Isinuka sulfur pool remain poorly understood. Using high-throughput Illumina 16S rRNA sequencing and PICRUSt-based functional inference, we analyzed bacterial diversity and metabolic potential in sediment and water samples. Sediments, characterized by high sulfide/sulfate/thiosulfate, salinity, alkalinity, and organic matter content under anoxic conditions, supported diverse sulfur-reducing and organic-degrading bacteria, primarily from the Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria phyla. In contrast, the anoxic water column harbored a less diverse community dominated by α-, γ-, and β-Proteobacteria, including Thiocapsa and Lutimaribacter. Sulfur oxidation genes (soxABCXYZ, sqr) were abundant in water, while sulfate reduction genes (dsrAB, aprAB, and sat/met3) were concentrated in sediments. Core microbiome analysis identified Thiocapsa, Lutimaribacter, and Delftia as functional keystones, integrating sulfur oxidation and nutrient recycling. Sediments supported dissimilatory sulfate-reducing bacteria (unclassified Desulfobacteraceae, Desulfosarcina, Desulfococcus, Desulfotignum, and Desulfobacter), while water samples were enriched in sulfur-oxidizing bacteria like Thiocapsa. Metabolic profiling revealed extensive sulfur, nitrogen, and carbon cycling pathways, with sulfur autotrophic denitrification and anoxygenic photosynthesis coupling sulfur-nitrogen and sulfur-carbon cycles. This study provides key theoretical insights into the microbial dynamics in sulfur-rich environments, highlighting their roles in biogeochemical cycling and potential applications in environmental management.

Thiocapsa, Lutimaribacter和Delftia是促进南非Isinuka富硫化物泉中硫氧化和养分循环耦合的主要细菌分类群。
硫循环是一个基本的生物地球化学过程,但它在伊西努卡硫池等环境中的微生物基础仍然知之甚少。利用高通量Illumina 16S rRNA测序和基于picrust的功能推断,我们分析了沉积物和水样中的细菌多样性和代谢潜力。沉积物在缺氧条件下具有高硫化物/硫酸盐/硫代硫酸盐、盐度、碱度和有机物含量的特征,支持多种硫还原和有机降解细菌,主要来自变形菌门、厚壁菌门、拟杆菌门和放线菌门。相比之下,缺氧水体的群落多样性较低,主要由α-、γ-和β-变形菌属(包括Thiocapsa和Lutimaribacter)组成。硫氧化基因soxABCXYZ、sqr在水中富集,硫酸盐还原基因dsrAB、aprAB、sat/met3在沉积物中富集。核心微生物组分析发现Thiocapsa、Lutimaribacter和Delftia是整合硫氧化和养分循环的功能关键。沉积物支持异化硫酸盐还原细菌(未分类的desulobacteraceae, Desulfosarcina, Desulfococcus, desulfotium和desulobacter),而水样则富含硫氧化细菌,如Thiocapsa。代谢分析揭示了广泛的硫、氮和碳循环途径,硫自养反硝化和无氧光合作用耦合硫-氮和硫-碳循环。本研究为富硫环境中的微生物动力学提供了重要的理论见解,突出了它们在生物地球化学循环中的作用和在环境管理中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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