Polyhexamethylene Biguanide Nanoparticles Inhibit Biofilm Formation by Mastitis-Causing Staphylococcus aureus.

IF 2 2区农林科学 Q2 VETERINARY SCIENCES

Veterinary Sciences Pub Date : 2025-05-21 DOI:10.3390/vetsci12050507

Renata de Freitas Leite, Breno Luis Nery Garcia, Kristian da Silva Barbosa, Thatiane Mendes Mitsunaga, Carlos Eduardo Fidelis, Bruna Juliana Moreira Dias, Renata Rank de Miranda, Valtencir Zucolotto, Liam Good, Marcos Veiga Dos Santos

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引用次数: 0

Abstract

Staphylococcus aureus is a mastitis pathogen that compromises cow health and causes significant economic losses in the dairy industry. High antimicrobial resistance and biofilm formation by S. aureus limit the efficacy of conventional treatments. This study evaluated the potential of polyhexamethylene biguanide nanoparticles (PHMB NPs) against mastitis-causing S. aureus. PHMB NPs showed low toxicity to bovine mammary epithelial cells (MAC-T cells) at concentrations up to four times higher than the minimum inhibitory concentration (1 µg/mL) against S. aureus. In Experiment 1, PHMB NPs significantly reduced biofilm formation by S. aureus by 50% at concentrations ≥1 µg/mL, though they showed limited efficacy against preformed biofilms. In Experiment 2, using an excised teat model, PHMB NPs reduced S. aureus concentrations by 37.57% compared to conventional disinfectants (chlorhexidine gluconate, povidone-iodine, and sodium dichloroisocyanurate), though limited by short contact time. These findings highlight the potential of PHMB NPs for the control of S. aureus growth and biofilm formation.

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聚六亚甲基双胍纳米颗粒抑制引起乳腺炎的金黄色葡萄球菌形成生物膜。

金黄色葡萄球菌是一种乳腺炎病原体，危害奶牛的健康，给乳制品行业造成重大的经济损失。高抗菌素耐药性和金黄色葡萄球菌形成的生物膜限制了常规治疗的效果。本研究评估了聚六亚甲基二胍纳米颗粒（PHMB NPs）对引起乳腺炎的金黄色葡萄球菌的潜力。PHMB NPs对牛乳腺上皮细胞（MAC-T细胞）具有较低的毒性，浓度高达对金黄色葡萄球菌最低抑制浓度（1µg/mL）的4倍。在实验1中，PHMB NPs在浓度≥1µg/mL时可显著减少金黄色葡萄球菌形成的生物膜50%，尽管它们对预形成的生物膜的作用有限。在实验2中，使用切除的小鼠模型，与传统消毒剂（葡萄糖酸氯己定、聚维酮碘和二氯异氰脲酸钠）相比，PHMB NPs降低了金黄色葡萄球菌浓度37.57%，尽管接触时间短。这些发现强调了PHMB NPs在控制金黄色葡萄球菌生长和生物膜形成方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Sciences VETERINARY SCIENCES-

CiteScore

2.90

自引率

8.30%

发文量

612

审稿时长

6 weeks

期刊介绍： Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.