Fine Mapping Identifies Candidate Genes Associated with Swine Inflammation and Necrosis Syndrome.

IF 2 2区 农林科学 Q2 VETERINARY SCIENCES
Katharina Gerhards, Sabrina Becker, Josef Kühling, Joel Mickan, Mirjam Lechner, Hermann Willems, Gerald Reiner
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引用次数: 0

Abstract

Swine inflammation and necrosis syndrome (SINS) is a widespread disease in pigs, causing pain, suffering, and damage. Inflammation is documented at different levels based on clinical signs, histopathology, clinical chemistry, metabolomics and transcriptomics. The influence of sow and boar, as well as a heritability of around 0.3, suggest a genetic component to the disease. The aim of the present study was to identify functional single nucleotide polymorphisms (SNPs) in the vicinity of gene markers previously mapped using GWAS. DNA samples were available from 234 already phenotyped piglets. These animals were re-sequenced with additional prior enrichment. The nine selected chromosomal regions cover a total length of 22 Mbp. The genome-wide association study (GWAS) revealed two series with a total of 15 significant missense polymorphisms on chromosomes 11, 14, and 15. The homozygous genotypes of the most discriminating SNPs in series 1 resulted in SINS scores of 3.5 and 17.9, respectively. Despite the partial linkage of the SNPs, interesting candidate genes were defined. The results allow a significant narrowing of the possible candidate genes for understanding the pathogenesis of SINS and for future use in selection breeding to overcome the syndrome. Further studies should be carried out on larger animal populations.

精细定位确定与猪炎症和坏死综合征相关的候选基因。
猪炎症和坏死综合征(SINS)是一种在猪中广泛传播的疾病,可引起疼痛、痛苦和损害。根据临床体征、组织病理学、临床化学、代谢组学和转录组学,炎症在不同程度上被记录下来。母猪和公猪的影响,以及0.3左右的遗传率,表明这种疾病有遗传成分。本研究的目的是鉴定基因标记附近的功能性单核苷酸多态性(SNPs),之前使用GWAS定位。从234头已经表型化的仔猪中获得了DNA样本。这些动物被重新测序,并进行了额外的事前富集。所选的9个染色体区域的总长度为22 Mbp。全基因组关联研究(GWAS)揭示了在染色体11、14和15上的两个系列共15个显著错义多态性。系列1中最具鉴别性的snp纯合基因型的SINS得分分别为3.5和17.9。尽管这些snp存在部分连锁,但仍确定了有趣的候选基因。这些结果为了解SINS的发病机制和未来在选择育种中克服该综合征提供了可能的候选基因范围。应该对更大的动物种群进行进一步的研究。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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