Daniel Diniz da Gama, Gabriela Martines, Moacyr Rigueiro, Matheus Cruz, Paulo Schor
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引用次数: 0
Abstract
Purpose: To evaluate the clinical and histopathological performance of polydioxanone (PDO) membranes in conjunctival reconstruction compared with amniotic membrane (AM), assessing epithelialization, inflammation, and tissue integration in a rabbit model.
Methods: Fifteen New Zealand white rabbits underwent conjunctival resection, with each eye receiving either a PDO or AM graft. Animals were euthanized at 7, 14, 21, and 28 days. Clinical and histopathological evaluation included epithelialization, inflammation, fibrosis, granulation tissue, and graft retention.
Results: Both membranes supported conjunctival healing, with no statistically significant differences in epithelialization, inflammation, fibrosis, presence of granulation tissue, or graft remnants. PDO provided structured handling, whereas AM was more delicate but surgically challenging. Histopathology revealed similar inflammatory and regenerative responses, confirming PDO biocompatibility.
Conclusions: The PDO membrane is a viable synthetic alternative to AM for conjunctival reconstruction. Despite minor differences in handling and degradation, PDO exhibited comparable efficacy. Further human studies are needed to validate its application.
Translational relevance: These findings support the potential use of PDO membranes in ocular surface reconstruction, particularly in settings where AM availability is limited. The use of PDO could expand treatment options for conjunctival defects and enhance surgical outcomes in ophthalmology.
期刊介绍：
Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO.
The journal covers a broad spectrum of work, including but not limited to:
Applications of stem cell technology for regenerative medicine,
Development of new animal models of human diseases,
Tissue bioengineering,
Chemical engineering to improve virus-based gene delivery,
Nanotechnology for drug delivery,
Design and synthesis of artificial extracellular matrices,
Development of a true microsurgical operating environment,
Refining data analysis algorithms to improve in vivo imaging technology,
Results of Phase 1 clinical trials,
Reverse translational ("bedside to bench") research.
TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.
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