Michael Scheschenja, Jarmila Jedelská, Eva Juchems, Marc Weinmann, Axel Pagenstecher, Frederik Helmprobst, Malte Buchholz, Marina Tatura, Jens Schaefer, Udo Bakowsky, Alexander M König, Andreas H Mahnken
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引用次数: 0
Abstract
Background: The chick chorioallantoic membrane (CAM) model has been utilized for radiofrequency ablation and electroporation, but not yet for cryoablation. This study aims to evaluate the feasibility of the CAM model for preclinical cryoablation research.
Methods: Two cryoablation protocols were established for the study: 120 s-freeze-120 s-thaw-120 s freeze (120 s protocol) and 180 s-freeze-120 s-thaw-180 s freeze (180 s protocol). The study was divided into two parts. First, to evaluate embryo survival, fertilized chicken eggs were incubated. On embryonic day (ED) 12, cryoablation on CAM was performed according to the two protocols. During cryoablation, the temperature of the CAM was recorded using a thermal camera. Embryo survival was monitored until ED 14. Second, to evaluate tumor cryoablation, human neuroendocrine tumor cells (BON-1) were xenografted onto the CAM of fertilized chicken eggs at ED 8. Cryoablation of the xenografted tumors was then performed on ED 12 according to the two protocols. Ablation outcomes were evaluated by stereomicroscopic and histological assessments after harvesting on ED 14.
Results: Embryo survival rates were 8/9 in both protocols. A decrease in the peripheral temperature of 4.5 (± 0.9) °C and 6.7 (± 1.0) °C was observed in the 120 s and 180 s protocols, respectively. Complete ablation of CAM-grown tumors was observed in 2/6 (120 s protocol) and 2/5 (180 s protocol) cases, few scattered tumor cells remaining in 2/6 (120 s protocol) and 2/5 (180 s protocol) cases. Residual interconnected tumor cells were visible in 2/6 (120 s protocol) and 1/5 (180 s protocol) cases.
Conclusion: The CAM model is a feasible platform for preclinical cryoablation studies.
Relevance statement: Chorioallantoic membrane model is a suitable platform for preclinical cryoablation research.
Key points: Chick embryos tolerate the temperature drop during cryoablation well with high survival. Effectiveness of cryoablation on xenografted tumors can be histologically evaluated. Cryoablation protocols for xenografted tumors can be further optimized.
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