Toward Natural Wound Healing Therapy: Honey and Calendula officinalis Loaded κ-Carrageenan Films with Promising Hemostatic Potential.

IF 4.9 3区医学 Q1 PHARMACOLOGY & PHARMACY

Pharmaceutics Pub Date : 2025-04-28 DOI:10.3390/pharmaceutics17050578

Jovana S Vuković, Srđan Perišić, Anja Nikolić, Ivan Milošević, Milorad Mirilović, Bogomir Bolka Prokić, Tijana Lužajić Božinovski

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引用次数: 0

Abstract

Background/Objectives: Efficient wound treatment embraces the management of four overlapping phases, starting with hemostasis, an immediate physiological response aimed at stopping bleeding from damaged blood vessels caused by skin injury. This paper proposes an innovative, nature-based hemostatic biomaterial designed to assist natural self-healing regenerative mechanisms. Methods: Light, transparent, and skin-adhesive films based on κ-carrageenan, meadow polyfloral honey, and Calendula officinalis flower extract were fabricated via solution casting. Comprehensive characterization revealed the physicochemical, structural, swelling, and barrier properties and the influence of each bioactive compound utilized for film preparation. Results: The samples subcutaneously implanted in Wistar rats induced vascularization, deposition of collagen, and orientation of collagen fibers while being fully phagocytosed and gradually biodegraded. The rat tail-cut model demonstrated that the films significantly reduced blood loss (0.1875 ± 0.0732 g) compared to the control (0.7837 ± 0.3319 g), and hemostasis was achieved notably faster (355.75 ± 71.42 s) than in the control group (704.25 ± 85.29 s). The rat liver punch biopsy model confirmed reduced blood loss (2.8025 ± 1.5174 g) and shorter time to hemostasis (303.25 ± 77.90 s) compared to the control (3.1475 ± 1.5413 g, 383.00 ± 36.53 s). Conclusions: The results indicate the great potential of the fabricated films as hemostatic wound dressings.

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伤口自然愈合疗法：蜂蜜和金盏花负载κ-卡拉胶膜具有良好的止血潜力。

背景/目的：有效的伤口治疗包括四个重叠阶段的管理，从止血开始，这是一种即时的生理反应，旨在阻止皮肤损伤引起的血管损伤出血。本文提出了一种创新的，基于自然的止血生物材料，旨在帮助自然自愈再生机制。方法：以κ-卡拉胶、草甸多花蜂蜜、金盏花提取物为基材，采用溶液浇铸法制备轻薄透明的贴膜。综合表征揭示了用于薄膜制备的每种生物活性化合物的物理化学，结构，膨胀和屏障特性以及影响。结果：Wistar大鼠皮下植入的样品在被充分吞噬和逐渐生物降解的同时，诱导血管形成、胶原沉积和胶原纤维取向。大鼠切尾模型显示，与对照组（0.7837±0.3319 g）相比，膜组出血量（0.1875±0.0732 g）明显减少，止血速度（355.75±71.42 s）明显快于对照组（704.25±85.29 s）。大鼠肝脏穿刺活检模型证实，与对照组（3.1475±1.5413 g, 383.00±36.53 s）相比，大鼠失血量减少（2.8025±1.5174 g），止血时间缩短（303.25±77.90 s）。结论：制备膜作为止血创面敷料具有广阔的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

7.90

自引率

11.10%

发文量

2379

审稿时长

16.41 days

期刊介绍： Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications, and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.