Temporal Variation and Human Host Predominance in Aedes aegypti from Coastal and Western Kenya: Insights from Pooled Blood Meal Metagenomics.

IF 3.3 3区 医学 Q2 MICROBIOLOGY
Kavinya Mwendwa, Francis Mutuku, Sammy Wambua, Makenzi Nzaro, Bryson A Ndenga, Kennedy Agoi, Angelle D LaBeaud, Carren Bosire
{"title":"Temporal Variation and Human Host Predominance in <i>Aedes aegypti</i> from Coastal and Western Kenya: Insights from Pooled Blood Meal Metagenomics.","authors":"Kavinya Mwendwa, Francis Mutuku, Sammy Wambua, Makenzi Nzaro, Bryson A Ndenga, Kennedy Agoi, Angelle D LaBeaud, Carren Bosire","doi":"10.3390/pathogens14050505","DOIUrl":null,"url":null,"abstract":"<p><p><i>Aedes aegypti</i> is the primary vector of arboviral diseases such as dengue, chikungunya, yellow fever, and Zika, posing significant global health and economic challenges. The effective control of this mosquito species requires understanding its seasonality, feeding behavior, and ecological dynamics. Modern molecular techniques, such as amplicon metagenomic sequencing, provide insights into vector-host interactions and feeding patterns. This study investigated the temporal variation of <i>Ae. aegypti</i> abundance and its blood meal sources in coastal and western Kenya over 16 months. A total of 64,360 mosquitoes were collected, with <i>Ae. aegypti</i> comprising 10.9% (7035/64,360). Coastal sites had a higher proportion (64.7%) of <i>Ae. aegypti</i> than western Kenya. Seasonal variation in abundance was observed, with peaks during the long rainy season and decline during the dry season. Blood meal analysis identified 15 vertebrate hosts, with humans being the primary source (86.6-95.9%). Other hosts included domestic animals such as turkey, sheep, cow, goat, and chicken. These findings highlight the role of rainfall in arboviral disease transmission and <i>Ae. aegypti</i>'s strong preference for human hosts. Additionally, this study demonstrates the cost-effectiveness of group testing for identifying blood meal sources, with implications for public health interventions.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":"14 5","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114503/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathogens","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/pathogens14050505","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Aedes aegypti is the primary vector of arboviral diseases such as dengue, chikungunya, yellow fever, and Zika, posing significant global health and economic challenges. The effective control of this mosquito species requires understanding its seasonality, feeding behavior, and ecological dynamics. Modern molecular techniques, such as amplicon metagenomic sequencing, provide insights into vector-host interactions and feeding patterns. This study investigated the temporal variation of Ae. aegypti abundance and its blood meal sources in coastal and western Kenya over 16 months. A total of 64,360 mosquitoes were collected, with Ae. aegypti comprising 10.9% (7035/64,360). Coastal sites had a higher proportion (64.7%) of Ae. aegypti than western Kenya. Seasonal variation in abundance was observed, with peaks during the long rainy season and decline during the dry season. Blood meal analysis identified 15 vertebrate hosts, with humans being the primary source (86.6-95.9%). Other hosts included domestic animals such as turkey, sheep, cow, goat, and chicken. These findings highlight the role of rainfall in arboviral disease transmission and Ae. aegypti's strong preference for human hosts. Additionally, this study demonstrates the cost-effectiveness of group testing for identifying blood meal sources, with implications for public health interventions.

肯尼亚沿海和西部埃及伊蚊的时间变异和人类宿主优势:来自汇集血餐元基因组学的见解。
埃及伊蚊是登革热、基孔肯雅热、黄热病和寨卡等虫媒病毒性疾病的主要媒介,对全球卫生和经济构成重大挑战。有效控制该蚊种需要了解其季节性、摄食行为和生态动态。现代分子技术,如扩增子宏基因组测序,提供了对媒介-宿主相互作用和摄食模式的见解。本研究对伊蚊的时间变化进行了研究。16个月来,肯尼亚沿海和西部的埃及伊蚊丰度及其血粉来源。共捕获蚊虫64360只,其中伊蚊;埃及伊蚊占10.9%(7035/64,360)。沿海生境中伊蚊的比例较高(64.7%)。埃及伊蚊比肯尼亚西部多。丰度随季节变化,在漫长的雨季达到峰值,在旱季下降。血粉分析鉴定出15种脊椎动物宿主,人类是主要来源(86.6-95.9%)。其他的主人包括家禽,如火鸡、绵羊、牛、山羊和鸡。这些发现强调了降雨在虫媒病毒病传播和伊蚊传播中的作用。埃及伊蚊对人类宿主的强烈偏好。此外,本研究还证明了群体检测识别血粉来源的成本效益,这对公共卫生干预具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Pathogens
Pathogens Medicine-Immunology and Allergy
CiteScore
6.40
自引率
8.10%
发文量
1285
审稿时长
17.75 days
期刊介绍: Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信