Improvement of Cryopreservation and Production of Attenuated Babesia Parasites to Prevent Bovine Babesiosis.

IF 3.3 3区 医学 Q2 MICROBIOLOGY
Carmen Rojas-Martínez, José J Lira-Amaya, Massaro W Ueti, Roberto O Castañeda-Arriola, Julio V Figueroa Millán, Jesús A Álvarez Martínez
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Abstract

This study evaluated the effects of various concentrations of PVP-40 on the in vitro cryopreservation and recovery of Babesia bovis and Babesia bigemina. We also assessed a reduced dose of attenuated Babesia strains to determine its efficacy in preventing clinical disease. A microaerophilic stationary phase blood culture system was used to recover Babesia parasites that were cryopreserved in solutions with various PVP-40 concentrations and Babesia parasites in 20% PVP-40 were used to vaccinate naïve cattle. The animals were vaccinated intramuscularly with frozen parasites cryopreserved in 20% PVP-40, with a dose of either 1 × 108 or 1 × 107 erythrocytes infected with both attenuated B. bigemina and B. bovis produced from blood cultures. The control group received uninfected erythrocytes. During the vaccination, clinical parameters such as rectal temperature and hematocrit levels were unaffected. The animals were relocated to a farm in a Babesia hyperendemic area to test the efficacy of these live vaccines in controlling disease onset. Some vaccinated animals showed mild disease. In the vaccinated groups, parasites were detected in blood smears for only one day during the challenge. In contrast, the control group experienced fever for three consecutive days, a decline in hematocrit levels, and significant health deterioration. In this group, parasites were detected in smears for four consecutive days. All the animals in the control group required treatment to manage their high parasitemia and prevent mortality. In this study, we demonstrated that increasing the concentration of PVP-40 to cryopreserve parasites improved the recovery and proliferation of Babesia spp. in blood culture, and we also showed that when animals were vaccinated with cryopreserved, in vitro cultured, attenuated Babesia parasites in 20% PVP-40, they were effectively protected from severe clinical babesiosis.

预防牛巴贝斯虫病的低温保存和减毒巴贝斯虫寄生虫生产的改进。
本研究评价了不同浓度PVP-40对牛巴贝斯虫和双巴贝斯虫体外冷冻保存和恢复的影响。我们还评估了减少剂量的减毒巴贝斯虫菌株,以确定其预防临床疾病的功效。采用微嗜气固定相血液培养系统回收在不同PVP-40浓度的溶液中冷冻保存的巴贝斯虫寄生虫,并用20% PVP-40的巴贝斯虫寄生虫接种naïve牛。用20% PVP-40冻存的冷冻寄生虫给动物肌肉接种,接种剂量分别为1 × 108或1 × 107红细胞,同时感染从血液培养中产生的减毒双头螺旋体和牛螺旋体。对照组给予未感染红细胞。在接种疫苗期间,临床参数如直肠温度和红细胞压积水平未受影响。这些动物被重新安置到巴贝斯虫高流行地区的一个农场,以测试这些活疫苗在控制疾病发病方面的功效。一些接种疫苗的动物表现出轻微的疾病。在接种疫苗的组中,寄生虫在血液涂片中只检测了一天。相比之下,对照组经历了连续三天的发烧,红细胞压积水平下降,健康状况明显恶化。在这一组中,连续4天在涂片中检测到寄生虫。对照组中的所有动物都需要治疗以控制其高寄生虫血症并防止死亡。在本研究中,我们证明了增加PVP-40的浓度来冷冻保存寄生虫,可以提高巴贝斯虫在血培养中的恢复和增殖,并且我们还表明,当动物接种冷冻保存的体外培养的减毒巴贝斯虫寄生虫时,20%的PVP-40可以有效地保护它们免受严重的临床巴贝斯虫病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Pathogens
Pathogens Medicine-Immunology and Allergy
CiteScore
6.40
自引率
8.10%
发文量
1285
审稿时长
17.75 days
期刊介绍: Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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