Intestinal microbiome profile of the brown rock sea cucumber (Holothuria glaberrima) using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction.
Rene Nieves-Morales, Jessica Alejandra Paez-Diaz, Sofia Marie Rodriguez-Carrio, Gabriela Melendez Martinez, Edwin Omar Rivera-Lopez, Josué Rodríguez-Ramos, José E García-Arrarás, Carlos Rios-Velazquez
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Abstract
Using direct mechanical, enzymatic, and chemical extraction methods, the intestinal microbiome of the marine invertebrate Holothuria glaberrima was obtained. ITS and 16S rDNA regions were sequenced to enrich and investigate the prokaryotic and fungal diversity profiles from different anatomical regions within the sea cucumber's intestinal biology.
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