The Characterization of Serum-Free Media on Human Mesenchymal Stem Cell Fibrochondrogenesis.

IF 3.8 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Ka Yu Carissa Kwan, Ke Li, Yu Yang Wang, Wai Yi Tse, Chung Yan Tong, Xu Zhang, Dan Michelle Wang, Dai Fei Elmer Ker
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引用次数: 0

Abstract

Developing fibrochondrogenic serum-free media is important for regenerating diseased and injured fibrocartilage but no defined protocols exist. Towards this goal, we characterized the effect of four candidate fibrochondrogenic serum-free media containing transforming growth factor beta-3 (TGF-β3), insulin-like growth factor-1 (IGF-1), and fibroblast growth factor-2 (FGF-2) with high/low glucose and with/without dexamethasone on human mesenchymal stem cells (hMSCs) via proliferation and differentiation assays. In Ki67 proliferation assays, serum-free media containing low glucose and dexamethasone exhibited the highest growth. In gene expression assays, serum-free media containing low glucose and commercially available chondrogenic media (COM) induced high fibrochondrogenic transcription factor expression (scleraxis/SCX and SRY-Box Transcription Factor 9/SOX9) and extracellular matrix (ECM) protein levels (aggrecan/ACAN, collagen type I/COL1A1, and collagen type II/COL2A1), respectively. In immunofluorescence staining, serum-free media containing high glucose and COM induced high fibrochondrogenic transcription factor (SCX and SOX9) and ECM protein (COL1A1, COL2A1, and collagen type X/COL10A1) levels, respectively. In cytochemical staining, COM and serum-free media containing dexamethasone showed a high collagen content whereas serum-free media containing high glucose and dexamethasone exhibited high glycosaminoglycan (GAG) levels. Altogether, defined serum-free media containing high glucose exhibited the highest fibrochondrogenic potential. In summary, this work studied conditions conducive for fibrochondrogenesis, which may be further optimized for potential applications in fibrocartilage tissue engineering.

无血清培养基对人间充质干细胞成纤维软骨的影响。
发展无血清纤维软骨生成介质对再生病变和损伤的纤维软骨是重要的,但没有明确的方案存在。为了实现这一目标,我们通过增殖和分化实验研究了四种候选的无血清纤维软骨生成培养基,这些培养基含有转化生长因子β -3 (TGF-β3)、胰岛素样生长因子-1 (IGF-1)和成纤维细胞生长因子-2 (FGF-2),含高/低糖和含/不含地塞米松对人间充质干细胞(hMSCs)的影响。在Ki67增殖试验中,含低糖和地塞米松的无血清培养基表现出最高的生长。在基因表达试验中,含低糖的无血清培养基和市售的软骨培养基(COM)分别诱导高纤维软骨生成转录因子(sclcleraxis /SCX和SRY-Box转录因子9/SOX9)和细胞外基质(ECM)蛋白(aggrecan/ACAN、I型胶原/COL1A1和II型胶原/COL2A1)表达。在免疫荧光染色中,含高糖和COM的无血清培养基分别诱导高纤维软骨生成转录因子(SCX和SOX9)和ECM蛋白(COL1A1、COL2A1和X型胶原/COL10A1)水平。在细胞化学染色中,COM和含地塞米松的无血清培养基中胶原蛋白含量高,而含高葡萄糖和地塞米松的无血清培养基中糖胺聚糖(GAG)含量高。总之,含有高葡萄糖的无血清培养基显示出最高的纤维软骨形成潜力。综上所述,本工作研究了有利于纤维软骨形成的条件,可以进一步优化其在纤维软骨组织工程中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bioengineering
Bioengineering Chemical Engineering-Bioengineering
CiteScore
4.00
自引率
8.70%
发文量
661
期刊介绍: Aims Bioengineering (ISSN 2306-5354) provides an advanced forum for the science and technology of bioengineering. It publishes original research papers, comprehensive reviews, communications and case reports. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. All aspects of bioengineering are welcomed from theoretical concepts to education and applications. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, four key features of this Journal: ● We are introducing a new concept in scientific and technical publications “The Translational Case Report in Bioengineering”. It is a descriptive explanatory analysis of a transformative or translational event. Understanding that the goal of bioengineering scholarship is to advance towards a transformative or clinical solution to an identified transformative/clinical need, the translational case report is used to explore causation in order to find underlying principles that may guide other similar transformative/translational undertakings. ● Manuscripts regarding research proposals and research ideas will be particularly welcomed. ● Electronic files and software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. ● We also accept manuscripts communicating to a broader audience with regard to research projects financed with public funds. Scope ● Bionics and biological cybernetics: implantology; bio–abio interfaces ● Bioelectronics: wearable electronics; implantable electronics; “more than Moore” electronics; bioelectronics devices ● Bioprocess and biosystems engineering and applications: bioprocess design; biocatalysis; bioseparation and bioreactors; bioinformatics; bioenergy; etc. ● Biomolecular, cellular and tissue engineering and applications: tissue engineering; chromosome engineering; embryo engineering; cellular, molecular and synthetic biology; metabolic engineering; bio-nanotechnology; micro/nano technologies; genetic engineering; transgenic technology ● Biomedical engineering and applications: biomechatronics; biomedical electronics; biomechanics; biomaterials; biomimetics; biomedical diagnostics; biomedical therapy; biomedical devices; sensors and circuits; biomedical imaging and medical information systems; implants and regenerative medicine; neurotechnology; clinical engineering; rehabilitation engineering ● Biochemical engineering and applications: metabolic pathway engineering; modeling and simulation ● Translational bioengineering
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