A ratio fluorescence/colorimetry/smartphone triple-mode sensor based on solvent-dominated signal amplification strategy for precise and rapid detection of melatonin in fruit juice

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-05-28 DOI:10.1007/s00604-025-07248-0

Linjie Wang, Lu Wang, Lin Jing, Yang Yang, Hui Miao, Xiaoya Liu, Dongzhi Suonanmu, Qingjia Ren, Fei Wang, Caolong Li

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引用次数: 0

Abstract

A ratio fluorescence/colorimetry/smartphone triple-mode sensor based on Ru-C₃N₄ was established for precise and rapid detection of melatonin (MT). Specifically, Ru-C₃N₄ with fine luminous characteristic and peroxidase-like activity catalyzes the oxidation of OPD to generate luminescent DAP which in turn suppresses intrinsic fluorescence of Ru-C₃N₄ because of the fluorescence inner filter effect (IFE). MT can inhibit this catalytic process, leading to partial recovery of the fluorescence of Ru-C₃N₄ and the decrease in UV–vis signal of the reaction system, as well as the gradual fading of the yellow color of the reaction solution. Hence, this triple-mode sensor can realize precise, reliable, and high-throughput determination of MT with correlative detection limits of 0.32, 0.46, and 0.67 μM. Moreover, this triple-mode sensor has been applied to MT detection in multiple juice samples with recoveries ranging from 86.00 to 99.68%, which indicates the suitability of the novel idea for the research and development of multimode optical sensors for food analysis.

Graphical Abstract

查看原文本刊更多论文

基于溶剂主导信号放大策略的比例荧光/比色法/智能手机三模传感器用于果汁中褪黑素的精确快速检测。

建立了一种基于Ru-C3N4的比例荧光/比色法/智能手机三模传感器，用于精确、快速检测褪黑素（MT）。具体来说，具有优良发光特性和过氧化物酶样活性的Ru-C3N4催化OPD氧化生成发光DAP，而发光DAP又由于荧光内滤效应（IFE）抑制了Ru-C3N4的固有荧光。MT可以抑制这一催化过程，导致Ru-C3N4的荧光部分恢复，反应体系的UV-vis信号减弱，反应溶液的黄色逐渐褪色。因此，该三模传感器可实现MT的精确、可靠、高通量测定，相关检出限分别为0.32、0.46和0.67 μM。此外，该三模传感器已应用于多种果汁样品的MT检测，回收率在86.00 ~ 99.68%之间，表明该新思路适合用于食品分析的多模光学传感器的研究和开发。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.