Decreased DMI sensitivity of Plenodomus biglobosus (phoma of oilseed rape) associated with CYP51 substitution G476S.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2025-05-28 DOI:10.1002/ps.8926

Kevin M King, Liza M González-Rodríguez, Joanna Kaczmarek, Małgorzata Jędryczka, Jonathan S West

{"title":"Decreased DMI sensitivity of Plenodomus biglobosus (phoma of oilseed rape) associated with CYP51 substitution G476S.","authors":"Kevin M King, Liza M González-Rodríguez, Joanna Kaczmarek, Małgorzata Jędryczka, Jonathan S West","doi":"10.1002/ps.8926","DOIUrl":null,"url":null,"abstract":"Background: Phoma leaf spot/stem canker is an international oilseed rape (Brassica napus) disease caused by Plenodomus lingam (Pl) and Plenodomus biglobosus (Pb). Phoma management can include fungicide applications, often sterol 14α-demethylase (CYP51) inhibitors (DMIs). Pl and Pb isolates (collected throughout Poland in 2024) were screened for in vitro sensitivity to the DMI prothioconazole-desthio, and molecular mechanisms associated with altered sensitivity were investigated.Results: Pl isolates (2024) were less sensitive (4-fold) than older (before 2002) isolates. All Pl (2024) isolates carried CYP51 promoter inserts, likely a Sahana transposable element (TE) fragment, previously associated with CYP51 overexpression and decreased DMI sensitivity. In addition, Pb isolates with decreased DMI sensitivity were also identified, but without such TE inserts. CYP51 gene sequencing instead revealed substitution G476S that was absent in more-sensitive G476 isolates. G476S homologues have been linked to decreased DMI sensitivity in multiple other fungi. Pb G476S isolates were less sensitive (7.3-fold) than Pb G476, widespread throughout Poland, and comprised 14 of 24 (58%) Pb isolates tested. Although CYP51 promoter inserts were detected in 2 of 24 (8%) Pb isolates, these were promoter sequence duplications (not TEs) and not obviously associated with decreased sensitivity. Pl isolates carrying promoter inserts (predominant in recently tested European populations) were more sensitive (2.6-fold) than Pb G476S, but less sensitive (2.8-fold) than Pb G476.Conclusion: We provide first evidence for a CYP51 substitution associated with decreased DMI sensitivity in Pb. Result implications for phoma management, how DMI usage might influence pathogen population structure, resistance management strategies and future research required are discussed. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pest Management Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/ps.8926","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Phoma leaf spot/stem canker is an international oilseed rape (Brassica napus) disease caused by Plenodomus lingam (Pl) and Plenodomus biglobosus (Pb). Phoma management can include fungicide applications, often sterol 14α-demethylase (CYP51) inhibitors (DMIs). Pl and Pb isolates (collected throughout Poland in 2024) were screened for in vitro sensitivity to the DMI prothioconazole-desthio, and molecular mechanisms associated with altered sensitivity were investigated.

Results: Pl isolates (2024) were less sensitive (4-fold) than older (before 2002) isolates. All Pl (2024) isolates carried CYP51 promoter inserts, likely a Sahana transposable element (TE) fragment, previously associated with CYP51 overexpression and decreased DMI sensitivity. In addition, Pb isolates with decreased DMI sensitivity were also identified, but without such TE inserts. CYP51 gene sequencing instead revealed substitution G476S that was absent in more-sensitive G476 isolates. G476S homologues have been linked to decreased DMI sensitivity in multiple other fungi. Pb G476S isolates were less sensitive (7.3-fold) than Pb G476, widespread throughout Poland, and comprised 14 of 24 (58%) Pb isolates tested. Although CYP51 promoter inserts were detected in 2 of 24 (8%) Pb isolates, these were promoter sequence duplications (not TEs) and not obviously associated with decreased sensitivity. Pl isolates carrying promoter inserts (predominant in recently tested European populations) were more sensitive (2.6-fold) than Pb G476S, but less sensitive (2.8-fold) than Pb G476.

Conclusion: We provide first evidence for a CYP51 substitution associated with decreased DMI sensitivity in Pb. Result implications for phoma management, how DMI usage might influence pathogen population structure, resistance management strategies and future research required are discussed. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

查看原文本刊更多论文

与CYP51取代G476S相关的油菜大叶斑瘤DMI敏感性降低。

背景：油菜叶斑/茎溃疡病是由plendomus lingam （Pl）和plendomus biglobosus （Pb）引起的一种国际性油菜病害。瘤的管理可以包括杀菌剂的应用，通常是固醇14α-去甲基化酶（CYP51）抑制剂（DMIs）。我们筛选了Pl和Pb分离株（于2024年在波兰各地收集）对DMI prothioconazole-desthio的体外敏感性，并研究了与敏感性改变相关的分子机制。结果：2024年的Pl分离株比2002年以前的老株敏感性低4倍。所有Pl（2024）分离株都携带CYP51启动子插入，可能是Sahana转座元件（TE）片段，先前与CYP51过表达和DMI敏感性降低有关。此外，还鉴定出对DMI敏感性降低的Pb分离株，但没有这样的TE插入。CYP51基因测序结果显示，在更敏感的G476分离株中不存在替代G476S。G476S同源物与其他多种真菌对DMI的敏感性降低有关。Pb G476S分离株的敏感性较Pb G476低（7.3倍），广泛分布于波兰，在24株Pb分离株中占14株（58%）。虽然在24株Pb分离株中有2株（8%）检测到CYP51启动子插入，但这些是启动子序列重复（不是TEs），与敏感性降低没有明显关联。携带启动子插入的Pl分离株（在最近检测的欧洲人群中占主导地位）比Pb G476的敏感性高（2.6倍），但比Pb G476的敏感性低（2.8倍）。结论：我们提供了CYP51取代与降低DMI对Pb的敏感性相关的第一个证据。结果对淋巴瘤管理的意义、DMI使用对病原菌种群结构的影响、耐药性管理策略和未来需要进行的研究进行了讨论。©2025作者。由John Wiley & Sons Ltd代表化学工业协会出版的《害虫管理科学》。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.