Evaluation of immune-related parameters in acellular and cellular fractions in male and female of Atlantic blue crabs (Callinectes sapidus)

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-05-25 DOI:10.1016/j.fsi.2025.110456

Ricardo Gálvez, Francisco A. Guardiola

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引用次数: 0

Abstract

Brachyurans are relatively understudied compared to other decapods, with numerous fields remaining to be explored, including the immune response. Therefore, the aim of this study was to characterize several immune-related enzymes and activities of the Atlantic blue crab (Callinectes sapidus) in cell-free haemolymph (CFH) and in the haemocyte lysate supernatant (HLS), considering the gender factor and the suitability of measuring each activity in CFH or HLS. Data were expressed by millilitres of haemolymph or milligrams of protein to identify which of these units would be more robust and informative. For this, haemolymph of each gender was collected, and centrifuged to obtain CFH samples, whilst the cells were sonicated to get the HLS. Our results revealed that the protein concentration was higher in CFH compared to HLS. Considering milligrams of protein as units, phenoloxidase and esterase activities were lower in CFH regarding to HLS in both genders. Similarly, the lysozyme and phosphatase activities were lower in CFH compared to HLS but only in females. The values of lysozyme activity were higher in HLS of females compared to males. Studying millilitre of haemolymph as units, lysozyme activity exhibited higher activity in CFH compared to HLS in both genders, whilst phenoloxidase and esterase activities were higher in HLS, showing variations only in males. Interestingly, alkaline phosphatase activity was undetected in CFH male. Any variation was observed between the activities measured in both genders. Protease and antiprotease activities were higher and lower in CFH compared to HLS values, respectively, whilst any variation was observed between genders. Bactericidal activity against V. harveyi, V. alginolyticus and V. anguillarum exhibited stronger values in HLS than in CFH, independently of gender, as well as against P. damselae in females. Considering gender factor, the bactericidal activity against V. alginolyticus, V. anguillarum and P. damselae was higher in HLS of females than in males. However, the bactericidal activity against V. alginolyticus and P. damselae was higher in CFH of males compared to females. Our results could contribute to gender selection in future brachyurans studies considering the variability between males and females due to their distinct biology. Additionally, we provide data regarding the baseline values of several immune-related activities, establishing the most appropriate units from the biological point of view.

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大西洋蓝蟹（Callinectes sapidus）雄性和雌性非细胞和细胞部分免疫相关参数的评价

与其他十足类动物相比，短爪龙的研究相对不足，还有许多领域有待探索，包括免疫反应。因此，本研究的目的是表征大西洋蓝蟹（Callinectes sapidus）在无细胞血淋巴（CFH）和血细胞裂解液上清（HLS）中的几种免疫相关酶和活性，考虑性别因素以及在CFH或HLS中测量每种活性的适用性。数据以毫升血淋巴或毫克蛋白质表示，以确定哪一个单位更可靠，信息更丰富。为此，收集了每种性别的血淋巴，并将其离心以获得CFH样本，同时对细胞进行超声处理以获得HLS。结果表明，CFH的蛋白浓度高于HLS。以毫克蛋白质为单位，在CFH中酚氧化酶和酯酶活性比在HLS中低。同样，与HLS相比，CFH的溶菌酶和磷酸酶活性也较低，但仅限于雌性。溶菌酶的活性值在雌性的HLS中高于雄性。以毫升血淋巴为单位，溶菌酶活性在CFH中比在HLS中表现出更高的活性，而酚氧化酶和酯酶活性在HLS中更高，仅在男性中表现出差异。有趣的是，碱性磷酸酶活性在CFH男性中未检测到。在两性之间的活动测量中观察到任何差异。与HLS值相比，CFH的蛋白酶和抗蛋白酶活性分别较高和较低，而性别之间没有差异。对哈维弧菌、溶藻弧菌和鳗弧菌的抑菌活性在HLS中强于CFH（与性别无关），雌性对豆selae的抑菌活性也强于CFH。考虑到性别因素，对腐藻弧菌、鳗弧菌和少女弧菌的抑菌活性，雌性比雄性高。而对腐藻弧菌和豆selae病原菌的抑菌活性在雄CFH中高于雌CFH。我们的研究结果可能有助于未来短肢龙研究的性别选择，考虑到雄性和雌性之间由于其独特的生物学差异而存在的差异。此外，我们提供了关于几种免疫相关活动的基线值的数据，从生物学的角度建立了最合适的单位。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.