The presence of acylated homoserine lactones and diffusible signal factor in bronchoalveolar lavage fluid from horses with clinical exacerbation of severe equine asthma

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-05-26 DOI:10.1016/j.rvsc.2025.105720

Neza Mrzdovnik , Janja Babič , Dane Lužnik , Dušan Žigon , Matic Mrzdovnik , Gabrijela Tavčar-Kalcher , Viktorija Tomič , Francis Prescott John , Modest Vengust

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引用次数: 0

Abstract

Several bacteria associated with chronic lung pathology use quorum sensing (QS) signaling molecules to regulate their virulence in pure cultures and poly-microbial communities. Their excessive growth and biofilm formation in the respiratory tract increase the morbidity and mortality of inflammatory airway diseases in humans, such as chronic obstructive pulmonary disease (COPD), asthma and cystic fibrosis (CF). In horses, severe equine asthma (SEA) has many parallels to these human diseases. We hypothesized that QS molecules associated with the most common biofilm-forming lung pathogens in humans (Pseudomonas aeruginosa, Stenotrophomonas maltophilia) may also be present in the lungs of horses with SEA.

Samples of bronchoalveolar lavage fluid (BALf) were taken from twenty horses with exacerbated SEA. Microbiological cultures of the BALf samples were performed. Liquid chromatography coupled with tandem mass spectrometry was used to identify C4-HSL, C6-HSL, 3-oxo-C12-HSL and 11-methyl-2-dodecenoic acid, which are associated with the QS mechanisms of Pseudomonas aeruginosa and Stenotrophomonas maltophilia.

Stenotrophomonas maltophilia was identified in three horses. Pseudomonas aeruginosa was not identified in any sample. The quorum sensing molecules C4-HSL, C6-HSL, 3-oxo-C12-HSL associated with biofilm formation by P. aeruginosa and 11-methyl-2-dodecenoic acid associated with biofilm formation by S. maltophila were not detected.

It is unlikely that biofilm-forming bacterial strains associated with chronic lung disease in humans express similar virulence in SEA.

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重症马哮喘临床加重马支气管肺泡灌洗液中乙酰化同丝氨酸内酯和弥散信号因子的存在

几种与慢性肺病理相关的细菌在纯培养物和多微生物群落中使用群体感应（QS）信号分子来调节其毒力。它们在呼吸道的过度生长和生物膜的形成增加了人类炎症性气道疾病的发病率和死亡率，如慢性阻塞性肺疾病（COPD）、哮喘和囊性纤维化（CF）。在马身上，严重马哮喘（SEA）与这些人类疾病有许多相似之处。我们假设，与人类最常见的形成生物膜的肺部病原体（铜绿假单胞菌、嗜麦芽窄养单胞菌）相关的QS分子也可能存在于患有SEA的马的肺部。从20匹加重SEA的马身上采集支气管肺泡灌洗液（BALf）样本。对半数样品进行微生物培养。采用液相色谱联用串联质谱法鉴定了与铜绿假单胞菌和嗜麦芽寡养单胞菌QS机制相关的C4-HSL、C6-HSL、3-氧- c12 - hsl和11-甲基-2-十二烯酸。在3匹马中鉴定出嗜麦芽窄养单胞菌。未检出铜绿假单胞菌。未检测到铜绿假单胞菌形成生物膜相关的群体感应分子C4-HSL、C6-HSL、3-氧- c12 - hsl和嗜麦芽假单胞菌形成生物膜相关的11-甲基-2-十二烯酸。与人类慢性肺部疾病相关的生物膜形成细菌菌株不太可能在SEA中表达类似的毒力。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.