Screening and Discovery of Volatile Allergenic Components from Artemisia Ordosica in Yulin Region of Northern China

IF 12.2 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-05-28 DOI:10.1016/j.jhazmat.2025.138759

Xiaoxue Mo, Cheng Wang, Yi Shan, Siqi Wang, Wen Yang, Zhaomin Xia, Zhanguo Qiao, Tao Zhang, Yuanyuan Ding, Rui Liu, Huaizhen He, Langchong He

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Abstract

Allergic diseases are on the rise globally, and Mas-related G protein-coupled receptor X2 (MRGPRX2) has been identified as a key receptor mediating allergic diseases and pseudo-allergic reaction. The artemisia ordosica and other artemisia species are widely distributed in the Northern Hemisphere, and has been demonstrated a strong association with allergic diseases, yet the specific allergenic substances are unclear. This study investigated artemisia ordosica as potential source of allergens. Combining the MRGPRX2-His-tag@VS/CMC model with gas chromatography–mass spectrometry (GC–MS) analysis, five volatile components were identified as allergenic substances, including α-curcumene, (1S)-(-)-α-pinene, (1 R)-(+)-α-pinene, (-)-β-pinene, and (R)-(+)-limonene. Among these, α-Curcumene, a relatively abundant compound, exhibited the strongest allergenic activity both in vitro and in vivo models, inducing the release of β-hexosaminidase, histamine, and cytokines from mast cells in a dose-dependent manner. Furthermore, both the identified mixture of allergenic components and α-curcumene alone exhibited comparable allergenic activity to the artemisia ordosica extract in both local and systemic allergic reactions in mice. Notably, the total content of volatile allergenic components increased gradually with plant growth, reaching substantially higher levels in August and September, coinciding with higher average temperatures. To the best of our knowledge, this study is the first to identify the volatile allergenic components in artemisia ordosica and reveal their content variation throughout its growth cycle. Given the widespread distribution of artemisia species in various regions of the Northern Hemisphere and the shared presence of volatile allergenic components, these findings offer a valuable experimental basis for the prevention and treatment of allergic diseases in areas where artemisia species are cultivated.

Synopsis

This study highlights the importance of investigating the volatile allergenic components of artemisia ordosica in the Yulin region. The identification of these allergenic components warrants further attention to the potential health risks associated with artemisia ordosica, especially concerning allergic diseases

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玉林地区油蒿挥发性致敏成分的筛选与发现

全球变应性疾病呈上升趋势，mas相关G蛋白偶联受体X2 （MRGPRX2）已被确定为介导变应性疾病和伪过敏反应的关键受体。油蒿和其他种类的青蒿广泛分布于北半球，并已被证明与过敏性疾病密切相关，但具体的致敏物质尚不清楚。本研究探讨了油蒿作为过敏原的潜在来源。结合MRGPRX2-His-tag@VS/CMC模型和气相色谱-质谱（GC-MS）分析，鉴定出5种挥发性成分α-姜黄烯、(1S)-(-)-α-蒎烯、(1r)-(+)-α-蒎烯、(-)-β-蒎烯和(R)-(+)-柠檬烯为致敏物质。其中，α-姜黄烯含量相对丰富，在体外和体内模型中均表现出最强的致敏活性，诱导肥大细胞释放β-己糖氨酸酶、组胺和细胞因子，并呈剂量依赖性。此外，在小鼠的局部和全身过敏反应中，所鉴定的致敏成分混合物和α-姜黄素单独表现出与油蒿提取物相当的致敏活性。值得注意的是，随着植物生长，挥发性致敏成分的总含量逐渐增加，在8月和9月达到较高水平，与较高的平均气温相一致。据我们所知，本研究首次鉴定了油蒿中挥发性致敏成分，并揭示了其在整个生长周期中的含量变化。鉴于北半球不同地区的青蒿种类分布广泛，并且普遍存在挥发性致敏成分，这些发现为在种植青蒿种类的地区预防和治疗过敏性疾病提供了宝贵的实验基础。本研究强调了研究榆林地区油蒿挥发性致敏成分的重要性。这些致敏成分的鉴定值得进一步关注与油蒿有关的潜在健康风险，特别是与过敏性疾病有关的健康风险

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.