ERF.D2 negatively controls drought tolerance through synergistic regulation of abscisic acid and jasmonic acid in tomato

IF 10.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Journal Pub Date : 2025-05-27 DOI:10.1111/pbi.70157

Changan Zhu, Xinyan Li, Min Zhang, Shuwen Wang, Beiyu Jing, Chaoyi Hu, Hannah Rae Thomas, Yanhong Zhou, Jingquan Yu, Zhangjian Hu

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引用次数: 0

Abstract

SummaryPlants inevitably encounter a diverse array of constantly changing environmental stresses, and drought stands out as one of the most severe threats to plants. Abscisic acid (ABA) and jasmonic acid (JA) work synergistically to increase plant drought tolerance, but their interplay during drought response remains elusive. Here, we uncovered that ABA induced the degradation of a negative transcription regulator, ethylene responsive factor (ERF.D2), in tomato drought tolerance. We identified that ERF.D2 was phosphorylated at Ser‐52 by calcium‐dependent protein kinase 27 (CPK27) in an ABA‐dependent manner and underwent subsequent PUB22‐mediated ubiquitination. Degradation of ERF.D2 leads to the increase of the transcript levels of JA biosynthesis genes, allene oxide cyclase (AOC) and 12‐oxophytodienoic acid reductase 3 (OPR3), and endogenous concentration of JA, thus enhancing tomato plant drought tolerance. These findings demonstrate a novel insight into the molecular mechanism of ABA–JA synergistic interaction during tomato drought tolerance.

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小块土地。D2通过协同调控脱落酸和茉莉酸对番茄抗旱性产生负调控作用

植物不可避免地会遇到各种不断变化的环境压力，干旱是对植物最严重的威胁之一。脱落酸（ABA）和茉莉酸（JA）协同作用提高植物的抗旱性，但它们在干旱响应中的相互作用尚不清楚。在这里，我们发现ABA诱导了负转录调控因子乙烯响应因子（ERF.D2）在番茄抗旱性中的降解。我们确定了ERF。D2在Ser - 52位点被钙依赖蛋白激酶27 （CPK27）以ABA依赖的方式磷酸化，并随后经历PUB22介导的泛素化。ERF的退化。D2导致JA生物合成基因、烯氧化物环化酶（AOC）和12‐氧植物二烯酸还原酶3 （OPR3）的转录水平和内源JA浓度的增加，从而增强番茄植株的抗旱性。这些发现为番茄抗旱过程中ABA-JA协同作用的分子机制提供了新的见解。

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来源期刊

Plant Biotechnology Journal 生物-生物工程与应用微生物

CiteScore

20.50

自引率

2.90%

发文量

201

审稿时长

1 months

期刊介绍： Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.