Ultrasensitive Analysis of BRCA-1 Based on Gold Nanoparticles and Molybdenum Disulfide Electrochemical Immunosensor with Enhanced Signal Amplification.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Derya Bal Altuntaş
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Abstract

The BRCA-1 protein, recognized for its diagnostic relevance in a wide spectrum of malignancies, has been the focus of extensive investigation. In this study, an electrochemical immunosensor specifically designed for BRCA-1 detection was fabricated. The sensing platform utilizes disposable pencil graphite electrodes modified with a nanocomposite composed of gold nanoparticles (AuNPs), molybdenum disulfide (MoS2), and chitosan (CS). This multifunctional nanostructure significantly promotes electron transfer efficiency and supports the effective immobilization of antibodies. The constructed immunosensor exhibited excellent analytical performance, with a linear detection range between 0.05 and 20 ng/mL for BRCA-1 and a notably low limit of detection at 0.04 ng/mL. The device maintained a relative standard deviation of 3.59% (n = 3), indicating strong reproducibility. In addition, a high recovery rate of 98 ± 3% was achieved in spiked serum samples, even in the presence of common electroactive interferents such as dopamine and ascorbic acid. These findings highlight the sensor's promising applicability for the clinical detection of BRCA-1 and potentially other cancer-related biomarkers.

基于金纳米粒子和二硫化钼电化学免疫传感器增强信号放大的BRCA-1超灵敏分析。
BRCA-1蛋白被认为在广泛的恶性肿瘤中具有诊断意义,一直是广泛研究的焦点。本研究制作了一种专门用于BRCA-1检测的电化学免疫传感器。该传感平台采用一次性铅笔石墨电极,由金纳米粒子(AuNPs)、二硫化钼(MoS2)和壳聚糖(CS)组成的纳米复合材料修饰。这种多功能纳米结构显著提高了电子传递效率,并支持抗体的有效固定。所构建的免疫传感器具有良好的分析性能,对BRCA-1的线性检测范围为0.05 ~ 20 ng/mL,最低检测限为0.04 ng/mL。该装置的相对标准偏差为3.59% (n = 3),重复性强。此外,即使在常见的电活性干扰物如多巴胺和抗坏血酸存在的情况下,加标血清样品的回收率也达到98±3%。这些发现突出了该传感器在临床检测BRCA-1和其他潜在的癌症相关生物标志物方面的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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