Characterization of the complete mitochondrial genome of Monticola rufiventris and phylogenetic implications.

IF 3.8 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-05-27 DOI:10.1038/s41598-025-02187-5

Rui Hou, Quanfu Zhang, Mingna Duan, Shaobo Tang, Shuang Liu, Dandan Jiang, Chunhong Du, Xing Yang

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Abstract

Monticola rufiventris belongs to the order Passeriformes and the family Muscicapidae. As the largest group of birds, the phylogenetic relationships within Passeriformes have been a hot topic of research; however, studies on the genus Monticola within Passeriformes are relatively limited. This study presents the first comprehensive sequencing and analysis of the mitochondrial genome of M. rufiventris. Using Illumina Novaseq 6000 for paired-end sequencing, the results show that the mitochondrial genome of M. rufiventris is 16,803 bp, containing 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. Its structure is similar to that of known members of the Muscicapidae family, but there are some differences in gene size. Based on these data, we conducted mitochondrial assembly, selective pressure analysis, relative synonymous codon usage (RSCU) analysis, structural prediction of the 22 tRNAs, and phylogenetic tree construction. This study adds new data to the avian mitochondrial genome database, enhances the understanding of the Monticola mitochondrial genome, and provides valuable information for future research in avian taxonomy and phylogenetics.

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紫斑田鼠线粒体全基因组的特征及其系统发育意义。

Monticola rufiventris属于蝶形目和Muscicapidae科。雀形目作为鸟类中最大的类群，其系统发育关系一直是研究的热点。然而，对通道形目中Monticola属的研究相对有限。这项研究提出了第一个全面的测序和线粒体基因组分析M. rufiventris。利用Illumina Novaseq 6000对其线粒体基因组进行配对测序，结果显示，M. rufiventris线粒体基因组全长16803 bp，包含13个蛋白质编码基因、22个转移RNA基因、2个核糖体RNA基因和1个控制区。它的结构与已知的Muscicapidae家族成员相似，但在基因大小上存在一些差异。基于这些数据，我们进行了线粒体组装、选择压力分析、相对同义密码子使用（RSCU）分析、22个trna的结构预测和系统发育树构建。本研究为鸟类线粒体基因组数据库增加了新的数据，增强了对Monticola线粒体基因组的认识，为今后鸟类分类和系统发育研究提供了有价值的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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