OsSDG715, a Histone H3K9 Methyltransferase, Integrates Auxin and Cytokinin Signaling to Regulate Callus Formation in Rice.

IF 4.8 1区农林科学 Q1 AGRONOMY

Rice Pub Date : 2025-05-27 DOI:10.1186/s12284-025-00801-8

Wenjing Song, Hairong Cai, Yuanyuan Guo, Shiyi Chen, Yingyun Yao, Jiafeng Wang, Tao Guo, Jian Zhang, Chun Chen

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引用次数: 0

Abstract

Efficient callus induction is essential for the genetic transformation of rice (Oryza sativa), yet its regulatory mechanisms remain elusive. Previously, through a genome-wide association study (GWAS), we identified a significant associated locus on chromosome 8. In this study, we characterized this locus and demonstrated that OsSDG715, encoding a histone H3K9 methyltransferase, is the causal gene that positively regulates callus formation in rice. Results revealed that OsSDG715 is highly expressed during callus induction and exhibits natural variations associated with callus induction rate (CIR). Knockout of OsSDG715 via CRISPR/Cas9 led to a significant decrease in CIR and impaired callus morphology, indicating its positive regulation of callus formation. RNA-seq analyses revealed that 326 and 705 differentially expressed genes (DEGs) were upregulated and downregulated in sdg715 mutants, including auxin-responsive genes (OsIAA14, OsYUCCA6), cytokinin-related genes (OsCKX4, ARR10), and stress-responsive factors. Further analysis showed reduced endogenous indole-3-acetic acid (IAA) levels and increased zeatin levels in sdg715 mutants. These findings advance our understanding of the molecular mechanisms underlying rice callus formation, and offering valuable insights for optimizing tissue culture in molecular breeding.

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组蛋白H3K9甲基转移酶OsSDG715整合生长素和细胞分裂素信号调控水稻愈伤组织形成

有效的愈伤组织诱导是水稻遗传转化的关键，但其调控机制尚不明确。此前，通过全基因组关联研究（GWAS），我们在8号染色体上发现了一个重要的相关位点。在这项研究中，我们对该位点进行了表征，并证明编码组蛋白H3K9甲基转移酶的OsSDG715是水稻愈伤组织形成的正向调控基因。结果表明，OsSDG715在愈伤组织诱导过程中高表达，并随愈伤组织诱导率（CIR）的变化而发生自然变化。通过CRISPR/Cas9敲除OsSDG715导致CIR显著降低，愈伤组织形态受损，表明其对愈伤组织形成有正向调节作用。RNA-seq分析显示，326和705个差异表达基因（deg）在sdg715突变体中上调和下调，包括生长素反应基因（OsIAA14, OsYUCCA6），细胞分裂素相关基因（OsCKX4, ARR10）和应激反应因子。进一步分析表明，sdg715突变体内源吲哚-3-乙酸（IAA）水平降低，玉米素水平升高。这些发现促进了我们对水稻愈伤组织形成的分子机制的认识，并为分子育种中组织培养的优化提供了有价值的见解。

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来源期刊

Rice AGRONOMY-

CiteScore

10.10

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.