A Case Control Study to Assess Frequency, Risk Factors and Genetic Diversity of Entamoeba gingivalis.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-05-26 DOI:10.1111/odi.15391

Elahe Tahmasebi, Mohamad Ghanimatdan, Samira Hajisadeghi, Esmaeil Rafiei, Yosef Sharifi, Zahra Jafari Nodoushan, Leila Khedmat, Mohsen Rahimi

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引用次数: 0

Abstract

Objective: This case-control study aimed to investigate the prevalence of Entamoeba gingivalis subtypes ST1 and ST2 kamaktli in dental plaque samples from individuals referred to Shokri Dental Hospital in Tehran, Iran.

Methods: Fifty-three participants were selected for each group (periodontitis, gingivitis, and healthy control), resulting in a total of 159 dental plaque samples. The participants completed a questionnaire to assess risk factors for Entamoeba gingivalis infection. Total genomic DNA was extracted via a commercial kit. Entamoeba gingivalis subtypes were characterized using nested PCR and DNA sequencing.

Results: The overall prevalence of Entamoeba gingivalis was 17.6% (28/159). The periodontitis group had the highest prevalence (28.3%, 15/53), followed by gingivitis (17.0%, 9/53) and healthy individuals (7.5%, 4/53). Statistical analysis revealed that the prevalence of Entamoeba gingivalis was significantly greater in periodontal patients than in healthy individuals (p = 0.045). A lower level of education (p = 0.007) and lack of dental flossing (p = 0.000) were significantly associated with higher prevalence. Nested PCR and DNA sequencing identified Entamoeba gingivalis ST1 in 75% (21/28) and ST2-Kamaktli variants in 25% (7/28) of positive samples.

Conclusions: Higher Entamoeba gingivalis prevalence in periodontal disease suggests it may contribute to oral pathologies. Understanding risk factors like flossing can enhance prevention and treatment.

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评估牙龈内阿米巴发生频率、危险因素和遗传多样性的病例对照研究。

目的：本病例对照研究旨在调查来自伊朗德黑兰Shokri牙科医院的个人牙菌斑样本中牙龈内阿米巴ST1和ST2卡马克利亚型的流行情况。方法：每组53人（牙周炎组、牙龈炎组和健康对照组），共采集159个牙菌斑样本。参与者完成了一份评估牙龈内阿米巴感染危险因素的问卷。通过商用试剂盒提取总基因组DNA。采用巢式PCR和DNA测序对牙龈内阿米巴进行了分型鉴定。结果：龈内阿米巴总感染率为17.6%（28/159）。牙周炎组患病率最高（28.3%,15/53），牙龈炎组次之（17.0%,9/53），健康组次之（7.5%,4/53）。统计分析显示，牙周病患者的牙龈内阿米巴患病率明显高于健康人群（p = 0.045）。较低的教育水平（p = 0.007）和不使用牙线（p = 0.000）与较高的患病率显著相关。巢式PCR和DNA测序在75%（21/28）的阳性样本中鉴定出龈内阿米巴ST1变异，在25%（7/28）的阳性样本中鉴定出ST2-Kamaktli变异。结论：牙周病患者牙龈内阿米巴患病率较高，提示其可能与口腔病变有关。了解像使用牙线这样的危险因素可以加强预防和治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.