Identification and Genetic Analysis of Collinearity Loci for Interspecific Hybrid Sterility in Genus Oryza.

IF 4.8 1区农林科学 Q1 AGRONOMY

Rice Pub Date : 2025-05-27 DOI:10.1186/s12284-025-00803-6

Ying Yang, Qiuhong Pu, Yonggang Lv, Jing Li, Jiawu Zhou, Xianneng Deng, Xuanchen Song, Yu Zhang, Dayun Tao

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引用次数: 0

Abstract

Background: Hybrid sterility is a common phenomenon in hybrids between the Asian cultivated rice (Oryza sativa L.) and its relatives with AA genome, which limits the utilization of interspecific heterosis and favorable gene introgression. Numerous loci for hybrid sterility have been identified between O. sativa and its relatives. However, it remains elusive whether hybrid sterility between different species is controlled by a set of conserved loci, and whether there are variations in the genetic mode of these loci.

Results: In this study, six novel hybrid sterility loci for pollen sterility were identified from different cross combinations between O. sativa and its three wild relatives. S59 caused hybrid pollen sterility in hybrids between O. sativa and O. rufipogon. S60 and S61 controlled the hybrid pollen sterility between O. sativa and O. glumaepatula. S62, S63 and S64 governed the hybrid pollen sterility between O. sativa and O. barthii. Genetic and linkage analysis showed that S59, S60, and S62 were located in near the same region on the short arm of chromosome 5. S61 and S63 were mapped near RM27460 on the short arm of chromosome 12. S64 was restricted into the 60.27 kb region between RM4853 and RM3372 on the short arm of chromosome 3. The genetic behavior of six novel hybrid sterility loci follows one-locus allelic interaction model, the male gametes carrying the alleles of O. sativa in the heterozygotes were selectively aborted except for S62.

Conclusions: The findings from this research would provide a better understanding for the genetic nature of interspecific hybrid sterility in rice.

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稻属种间杂交不育共线性位点的鉴定与遗传分析。

背景：杂种不育是亚洲栽培稻（Oryza sativa L.）与AA基因组近缘品种杂交的普遍现象，这限制了品种间杂种优势和有利基因渗入的利用。水稻及其近缘种之间存在着大量的杂交不育位点。然而，不同物种间的杂交不育是否由一组保守的基因座控制，以及这些基因座的遗传模式是否存在差异，仍然是一个谜。结果：从玉米与3个野生近缘种的不同杂交组合中鉴定出6个新的花粉不育位点。S59对玉米杂交花粉不育有一定的影响。S60和S61控制玉米与玉米的杂交花粉不育性。S62、S63和S64控制着苜蓿与萱草杂交花粉的不育性。遗传和连锁分析表明，S59、S60和S62位于5号染色体短臂上的同一区域。S61和S63定位在12号染色体短臂RM27460附近。S64被限制在3号染色体短臂RM4853 ~ RM3372之间的60.27 kb区域。6个新型杂交不育位点的遗传行为遵循单位点等位基因互作模式，杂合子中除S62外，其余携带苜蓿等位基因的雄配子均选择性流产。结论：本研究为进一步认识水稻种间杂交不育的遗传性质提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rice AGRONOMY-

CiteScore

10.10

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.