Exploring the Mechanism of Luteolin in Protecting Chickens from Ammonia Poisoning Based on Proteomic Technology.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2025-05-14 DOI:10.3390/metabo15050326

Yu Jin, Azi Shama, Haojinming Tang, Ting Zhao, Xinyu Zhang, Falong Yang, Dechun Chen

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引用次数: 0

Abstract

Background: Ammonia (NH₃), a harmful gas, reduces livestock productivity, threatens their health, and causes economic losses. Luteolin (Lut), an anti-inflammatory flavonoid, may counteract these effects.

Methods: Our study explored luteolin's protective mechanisms on chicken splenic lymphocytes under ammonia stress using a simulation model and four-dimensional fast data-independent acquisition (4D-FastDIA) proteomics. We identified 316 proteins, with 69 related to ammonia's negative effects and 247 to Lut's protection. Thirty differentially expressed proteins (DEPs) were common to both groups, with 27 showing counter-regulation with Lut.

Results: Gene Ontology (GO) analysis showed DEPs enriched in molecular responses to interferons and the negative regulation of immune responses, mainly located extracellularly. Molecular function analysis revealed DEPs in antigen binding and synthase activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis linked DEPs to pathways like estrogen signaling, NOD-like receptor signaling, cytokine-cytokine receptor interaction, and JAK-STAT signaling. The quantitative real-time polymerase chain reaction (qRT-PCR) results indicated that the mRNA levels of Interferon Alpha and Beta Receptor subunit 2 (IFNAR2) and Signal Transducer and Activator of Transcription 1 (STAT1) were trending downward. This observation was in strong agreement with the downregulation noted in the proteomics analysis.

Conclusions: Lut's protective role against ammonia's adverse effects on chicken splenic lymphocytes is linked to the modulation of key signaling pathways, offering insights for further research on treating ammonia exposure with Lut.

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基于蛋白质组学技术探讨木犀草素对鸡氨中毒的保护作用机制。

背景：氨（NH3）是一种有害气体，会降低牲畜生产力，威胁其健康，并造成经济损失。木犀草素（Lut），一种抗炎类黄酮，可以抵消这些影响。方法：采用模拟模型和四维快速数据独立采集（4D-FastDIA）蛋白质组学方法，探讨木犀草素对氨胁迫下鸡脾淋巴细胞的保护机制。我们鉴定了316个蛋白质，其中69个与氨的负面影响有关，247个与Lut的保护有关。30个差异表达蛋白（DEPs）在两组中是共同的，27个与Lut表现出反调控。结果：基因本体论（Gene Ontology， GO）分析显示，DEPs富集于干扰素的分子应答和免疫应答的负调控，主要位于细胞外。分子功能分析显示DEPs与抗原结合和合酶活性有关。京都基因和基因组百科全书（KEGG）分析将DEPs与雌激素信号、nod样受体信号、细胞因子-细胞因子受体相互作用和JAK-STAT信号传导等途径联系起来。定量实时聚合酶链反应（qRT-PCR）结果显示，干扰素α和β受体亚基2 （IFNAR2）和信号传导与转录激活因子1 (STAT1) mRNA水平呈下降趋势。这一观察结果与蛋白质组学分析中发现的下调非常一致。结论：Lut对氨对鸡脾淋巴细胞的不良影响的保护作用与关键信号通路的调节有关，为进一步研究Lut治疗氨暴露提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.