Wharton's Jelly Bioscaffolds Improve Cardiac Repair with Bone Marrow Mononuclear Stem Cells in Rats.

IF 5 3区医学 Q1 ENGINEERING, BIOMEDICAL

Journal of Functional Biomaterials Pub Date : 2025-05-12 DOI:10.3390/jfb16050175

Luize Kremer Gamba, Laiza Kremer Gamba, Camila da Costa, Aline Luri Takejima, Rossana Baggio Simeoni, Isabella Cristina Mendes Rossa, Anna Clara Faidiga Silva, Julia Letícia de Bortolo, Marcos Antônio Denk, Seigo Nagashima, Carlos de Almeida Barbosa, Paulo Cesar Lock Silveira, Júlio César Francisco, Luiz César Guarita-Souza

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Abstract

This study assessed the impact of implanting mononuclear stem cells and Wharton's Jelly (WJ), either separately or together, on left ventricular dysfunction following myocardial infarction in Wistar rats. Functional and histopathological parameters were analyzed, and a rat model of left anterior descending coronary artery ligation was used. Treatments included an intramyocardial injection of 0.9% sodium chloride (control, n = 14), decellularized WJ (n = 12), bone marrow-derived mononuclear cells (BMMC) (n = 12), and bone marrow-derived mononuclear cells (BMMC) combined with WJ (n = 15). Echocardiography assessed the left ventricular function and ejection fraction over four weeks. Histological and immunohistochemical analyses with anti-factor VIII evaluated angiogenesis and collagen types I and III. The results showed no statistically significant effect on ventricular remodeling 30 days post-acute myocardial infarction (AMI). Moreover, the infarct area was significantly smaller in the BMMC + WJ group compared to the control group, suggesting a potential benefit in reducing myocardial scarring. BMMC + WJ therapy demonstrated potential for functional improvement and infarct size reduction 30 days post-infarction. Further studies are needed to confirm its therapeutic benefits.

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华顿果冻生物支架用大鼠骨髓单核干细胞改善心脏修复。

本研究评估了单个核干细胞和华氏果冻（WJ）分别或一起植入对Wistar大鼠心肌梗死后左心室功能障碍的影响。采用大鼠左冠状动脉前降支结扎术，分析其功能和组织病理学参数。治疗包括心肌内注射0.9%氯化钠（对照组，n = 14）、去细胞化WJ （n = 12）、骨髓源性单个核细胞（n = 12）和骨髓源性单个核细胞（BMMC）联合WJ （n = 15）。超声心动图评估左心室功能和射血分数超过四周。组织和免疫组织化学分析与抗因子VIII评估血管生成和胶原I型和III型。结果显示，急性心肌梗死（AMI）后30天心室重构无统计学意义。此外，与对照组相比，BMMC + WJ组的梗死面积明显更小，这表明BMMC + WJ在减少心肌瘢痕形成方面有潜在的益处。BMMC + WJ治疗在梗死后30天显示出功能改善和梗死面积缩小的潜力。需要进一步的研究来证实其治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Functional Biomaterials Engineering-Biomedical Engineering

CiteScore

4.60

自引率

4.20%

发文量

226

审稿时长

11 weeks

期刊介绍： Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.