Ceftaroline Exhibits Promising In Vitro Activity Against Methicillin-Resistant Staphylococcus aureus Clinical Isolates From Alexandria, Egypt.

IF 2.8 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2025-05-19 eCollection Date: 2025-01-01 DOI:10.1155/ijm/4558662
Hussien H Sallam, Asmaa A Ramadan, Nancy M Attia, Amira ElBaradei, Sherine M Shawky, Mohammed A El-Kholy
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引用次数: 0

Abstract

Introduction: Ceftaroline is a fifth-generation cephalosporin that was recently introduced into the Egyptian market for the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections. However, limited data are available regarding the susceptibility of MRSA isolates in Egypt to this antibacterial agent. This study aimed to determine the susceptibility of MRSA strains recovered from different clinical samples to ceftaroline and to investigate the prevalence of the mecA and mecC resistance genes. Methods: A total of 412 MRSA isolates were selected from 520 Staphylococcus aureus (S. aureus) samples. Identification and antibiotic susceptibility testing were performed using the VITEK-2 compact system. Molecular identification of the nuc gene, encoding nuclease enzyme, a species-specific marker for S. aureus, and the mecA and mecC genes associated with methicillin resistance was performed using the polymerase chain reaction (PCR) technique. Moreover, the in vitro activity of ceftaroline was explored using the disc diffusion method, and its minimum inhibitory concentration (MIC) was determined according to the Clinical Laboratory Standards Institute (CLSI) criteria. Staphylococcal Protein A (spa) typing was carried out for ceftaroline nonsusceptible strains as determined by MIC. Results: Most isolates were recovered from skin and soft tissue infections. Of the 412 clinical isolates, 407 (98.7%) were susceptible to ceftaroline, with an MIC of ≤ 1 mg/L, while five isolates (1.3%) showed a susceptible dose-dependent (SDD) profile with MIC values of 2-4 μg/mL. No isolates were resistant to ceftaroline. All isolates carried the nuc gene, 94% harbored mecA, while mecC was undetected. Of the five SDD isolates, three were identified as spa type t037, corresponding to ST-239, ST-240, or ST-241 by multilocus sequence type (MLST), whereas the two remaining isolates were untypeable. Conclusions: From various clinical samples, ceftaroline demonstrated excellent in vitro activity against MRSA strains, positioning it as a promising therapeutic option for managing MRSA infections in Egypt.

头孢他林对埃及亚历山大耐甲氧西林金黄色葡萄球菌临床分离株显示出良好的体外活性。
头孢他林是第五代头孢菌素,最近被引入埃及市场,用于治疗耐甲氧西林金黄色葡萄球菌(MRSA)感染。然而,关于埃及MRSA分离株对该抗菌剂的敏感性的数据有限。本研究旨在检测不同临床样本中回收的MRSA菌株对头孢他林的敏感性,并调查mecA和mecC耐药基因的流行情况。方法:从520份金黄色葡萄球菌(S. aureus)样本中筛选出412株MRSA。使用VITEK-2紧凑系统进行鉴定和药敏试验。利用聚合酶链反应(PCR)技术对金黄色葡萄球菌核酸酶编码基因nuc和甲氧西林耐药相关基因mecA、mecC进行了分子鉴定。采用圆盘扩散法考察头孢他林的体外活性,并按照美国临床实验室标准协会(CLSI)标准测定其最低抑菌浓度(MIC)。采用MIC法对头孢他林不敏感菌株进行葡萄球菌蛋白A (spa)分型。结果:大部分分离株均来源于皮肤和软组织感染。412株临床分离株中,407株(98.7%)对头孢他林敏感,MIC值≤1 mg/L, 5株(1.3%)呈剂量依赖性(SDD), MIC值为2 ~ 4 μg/mL。没有菌株对头孢他林耐药。所有分离株均携带nuc基因,94%携带mecA,未检出mecC。5株SDD分离株中,3株经多位点序列型(MLST)鉴定为spa型t037,分别为ST-239、ST-240和ST-241,其余2株未分型。结论:从各种临床样本中,头孢他林显示出出色的抗MRSA菌株的体外活性,使其成为埃及MRSA感染的有希望的治疗选择。
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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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