Mechanism Study on the Preventive Effect of ELITEA Compound Tea on Hyperuricemia in Rats Based on Serum Untargeted Metabolomics.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2025-05-19 DOI:10.3390/metabo15050336

Shulian Liu, Yongliang Zhu, Wenjiong Wang, Xianghan Zhang, Linrui Gao, Xiangjun Qiu

{"title":"Mechanism Study on the Preventive Effect of ELITEA Compound Tea on Hyperuricemia in Rats Based on Serum Untargeted Metabolomics.","authors":"Shulian Liu, Yongliang Zhu, Wenjiong Wang, Xianghan Zhang, Linrui Gao, Xiangjun Qiu","doi":"10.3390/metabo15050336","DOIUrl":null,"url":null,"abstract":"Background/Objectives: Hyperuricemia (HUA) has become the second largest metabolic disease after diabetes, and has become a major public health problem. The ELITEA compound tea extract can effectively reduce the serum uric acid level in HUA rat models. In this study, the mechanism of ELITEA compound tea on HUA was analyzed through serum untargeted metabolomics analysis. Methods: The rat model of HUA was established by feeding rats with a high uric acid diet. A total of 24 male SD rats were divided into a blank control group, a hyperuricemia model group, and an ELITEA compound tea prevention experimental group. UPLC-MS/MS was used to detect changes in metabolites in the blood of the three groups of rats, in order to identify potential biomarkers and study the mechanism of ELITEA compound tea in preventing HUA. Results: The ELITEA compound tea exhibited significant preventive effects on HUA rats. The analysis results showed that after ELITEA combined tea intervention, the 257 metabolites downregulated in the HUA model group showed an upward trend. Meanwhile, the 115 metabolites upregulated in the HUA model group showed a decreasing trend. Six main enrichment pathways were obtained, including arginine biosynthesis, histidine metabolism, pyrimidine metabolism, tryptophan metabolism, vitamin B6 metabolism, arginine and proline metabolism. Conclusions: ELITEA compound tea can effectively reduce the serum uric acid levels in HUA model rats. Based on the in-depth analysis of untargeted metabolomics, ELITEA compound tea mainly regulates the arginine biosynthesis pathway by modulating three important metabolites, arginine, glutamate, and ornithine, to reduce serum uric acid.","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 5","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114111/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Metabolites","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/metabo15050336","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background/Objectives: Hyperuricemia (HUA) has become the second largest metabolic disease after diabetes, and has become a major public health problem. The ELITEA compound tea extract can effectively reduce the serum uric acid level in HUA rat models. In this study, the mechanism of ELITEA compound tea on HUA was analyzed through serum untargeted metabolomics analysis. Methods: The rat model of HUA was established by feeding rats with a high uric acid diet. A total of 24 male SD rats were divided into a blank control group, a hyperuricemia model group, and an ELITEA compound tea prevention experimental group. UPLC-MS/MS was used to detect changes in metabolites in the blood of the three groups of rats, in order to identify potential biomarkers and study the mechanism of ELITEA compound tea in preventing HUA. Results: The ELITEA compound tea exhibited significant preventive effects on HUA rats. The analysis results showed that after ELITEA combined tea intervention, the 257 metabolites downregulated in the HUA model group showed an upward trend. Meanwhile, the 115 metabolites upregulated in the HUA model group showed a decreasing trend. Six main enrichment pathways were obtained, including arginine biosynthesis, histidine metabolism, pyrimidine metabolism, tryptophan metabolism, vitamin B6 metabolism, arginine and proline metabolism. Conclusions: ELITEA compound tea can effectively reduce the serum uric acid levels in HUA model rats. Based on the in-depth analysis of untargeted metabolomics, ELITEA compound tea mainly regulates the arginine biosynthesis pathway by modulating three important metabolites, arginine, glutamate, and ornithine, to reduce serum uric acid.

查看原文本刊更多论文

基于血清非靶向代谢组学的ELITEA复方茶预防大鼠高尿酸血症机制研究。

背景/目的：高尿酸血症（HUA）已成为仅次于糖尿病的第二大代谢性疾病，成为重大的公共卫生问题。ELITEA复方茶提取物能有效降低HUA大鼠模型血清尿酸水平。本研究通过血清非靶向代谢组学分析，分析ELITEA复方茶对HUA的作用机制。方法：采用高尿酸饮食法建立大鼠HUA模型。将24只雄性SD大鼠分为空白对照组、高尿酸血症模型组和ELITEA复方茶预防实验组。采用UPLC-MS/MS检测三组大鼠血液中代谢物的变化，寻找潜在的生物标志物，研究ELITEA复方茶预防HUA的作用机制。结果：复方菁英茶对HUA大鼠有明显的预防作用。分析结果显示，ELITEA联合茶干预后，HUA模型组下调的257种代谢物呈上升趋势。同时，HUA模型组上调的115种代谢物呈下降趋势。得到了精氨酸生物合成、组氨酸代谢、嘧啶代谢、色氨酸代谢、维生素B6代谢、精氨酸和脯氨酸代谢6条主要富集途径。结论：ELITEA复方茶能有效降低HUA模型大鼠血清尿酸水平。基于非靶向代谢组学的深入分析，ELITEA复方茶主要通过调节精氨酸、谷氨酸、鸟氨酸三种重要代谢物来调节精氨酸生物合成途径，降低血清尿酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.