Bacteria in the Phylum Bacillota Degrade Nicotine Through the Variant of Pyridine and Pyrrolidine Pathway.

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Xiu-Ming Liu, Xuan Hai, Cong-Jian Li, Yu-Fan Han, Xing Chen, Yu-Jin Fan, Jian Wu, En-Min Zhou, Yuan-Dong Li, Ling-Ling Yang, Yan-Qing Duan, Xiao-Yang Zhi
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Abstract

Nicotine is a harmful contaminant primarily derived from the waste produced by tobacco manufacturing facilities. Many bacterial strains have been reported to degrade nicotine, indicating their potential application in removing nicotine from sewage for wastewater treatment. The microbial degradation of nicotine is a green, economical, and efficient strategy, leading to a significant interest in discovering novel nicotine-degrading bacterial isolates. In this study, we isolated and identified 192 strains from tobacco leaves, roots, and rhizosphere soil. Nicotine utilization assays showed that 64 out of 192 strains had the potential capability of nicotine degradation, primarily within the genera Bacillus, Peribacillus, and Priestia in phylum Bacillota. Comparative genome analysis identified genes related to the variant of pyridine and pyrrolidine pathway scattered throughout the genomes of novel Bacillota strains. Phylogenetic analysis indicated that essential genes from these strains were evolutionarily distinct from others, forming a monophyletic clade. Our observations revealed that nicotine degradation-related genes have relatively independent origins and complex evolutionary histories in the phylum Bacillota.

杆菌门细菌通过吡啶和吡咯烷途径的变异降解尼古丁。
尼古丁是一种有害污染物,主要来自烟草生产设施产生的废物。据报道,许多细菌菌株可以降解尼古丁,这表明它们在去除污水中尼古丁用于废水处理方面具有潜在的应用前景。微生物对尼古丁的降解是一种绿色、经济、高效的策略,这使得人们对发现新的尼古丁降解细菌分离物产生了极大的兴趣。本研究从烟草叶片、根和根际土壤中分离鉴定了192株菌株。烟碱利用试验表明,192个菌株中有64个具有潜在的烟碱降解能力,主要是杆菌门芽孢杆菌属、芽孢杆菌属和Priestia属。比较基因组分析发现,与吡啶和吡咯烷途径变异相关的基因分散在新的芽孢杆菌菌株的基因组中。系统发育分析表明,这些菌株的基本基因在进化上不同于其他菌株,形成了一个单系进化枝。结果表明,烟碱降解相关基因在芽孢杆菌门中具有相对独立的起源和复杂的进化历史。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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